Experimental Techniques

Protocols This category is for the sharing of experimental protocols.
Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research.
Animal Behavior and Models
Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.)
Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures:

About the Experimental Techniques category [Experimental Techniques] (2)
Best marker for 'activated' microglia - CD68? [Microscopy (IHC/ISH/ICC/In Vivo)] (4)
In Vivo Extracellular Recordings in Mouse Dorsal Horn - Anyone done it? [Electrophysiology] (1)
Visualizing calcium transients in DRG axons using GCaMP [Experimental Techniques] (1)
RNAscope (In situ hybridization) to study gene expression in pain-related tissues [Experimental Techniques] (18)
Ipsilateral vs. Contralateral (Within-subject) vs. Sham/Vehicle Control for Unilateral Pain Models [Experimental Techniques] (3)
Design and Construction of a Two-Temperature Preference Behavioral Assay for [Reagents and Equipment] (1)
Mechanical threshold testing after CFA in hindpaw -> Edema in mid hindpaw makes the mice less sensitive? [Animal Behavior and Models] (9)
Has anyone tried conditioned place aversion (CPA) with pain? [Animal Behavior and Models] (4)
Inhibitory Neurons in Lamina I-II [Microscopy (IHC/ISH/ICC/In Vivo)] (2)
Pax2 antibody help [Microscopy (IHC/ISH/ICC/In Vivo)] (7)
Sciatic Nerve Recordings - Tips and Tricks? [Experimental Techniques] (1)
Iba-1 and GFAP antibodies [Reagents and Equipment] (9)
Electronic von Frey [Animal Behavior and Models] (6)
Sall1 as a microglia marker for IHC or FACS. Anyone tried? [Experimental Techniques] (2)
SOX9 as an Astrocyte Marker: Has anyone used in IHC or FACS? [Experimental Techniques] (1)
QuPath: A useful tool for analyzing microscopy images (tissue especially) [Microscopy (IHC/ISH/ICC/In Vivo)] (2)
Drop (immersion) fixation vs. transcardial perfusion of DRG and Spinal Cord [Experimental Techniques] (5)
I want to trace sensory fibers into the spinal cord. Do any retrograde tracers fill axons, not just cell bodies? [Experimental Techniques] (5)
Tissue clearing (CLARITY, CUBIC, iDisco...) on DRG and Spinal Cord? [Experimental Techniques] (2)
Retrograde tracker compatible with ISH [Experimental Techniques] (3)
Optogenetic activation of proprioceptors? [Experimental Techniques] (1)
Intrathecal Bicuculline/Strychnine as a model of mechanical allodynia in mouse? [Experimental Techniques] (1)
Intrathecal catheter with port for multiple intrathecal deliveries in mouse? [Experimental Techniques] (1)
Making coronal sections of spinal cord [Experimental Techniques] (8)
Tester rating dependence in Mouse Behavior Assays (Up-down vs Frequency) [Experimental Techniques] (4)
Habituation Protocol for rodent Von Frey assay? [Animal Behavior and Models] (7)
Simplified Up-Down Method - (SUDO). Anyone use it? [Animal Behavior and Models] (7)
Pinprick Hyperalgesia - What stimulus? [Animal Behavior and Models] (2)
What rack and chambers do you use for Von Frey assay? [Animal Behavior and Models] (7)