Best slice harp for spinal slice electrophysiology?


#1

Anyone have a recommendation for a good commercial harp to hold down slices? I’ve made one of out of paperclip and stocking and it’s not stable enough. What do you do?
@liz @fmoehring @lfqueme


#2

For slices, we use a slice anchor from Warner Instruments:
https://warneronline.com/product_info.cfm?id=80&alpha=a&name=Slice%20Anchors%20%26%20Kits%20(Harps)

Specifically order number 64-1411, Model number SHD-22F/15. I think your ideal harp may depend on the size of your recording chamber so I’d take a look at their whole selection: https://warneronline.com/images/image_enlarge.cfm?image=Series%2020%20and%2040%20slice%20hold-downs


#3

Hey @liz and everyone else. An update. I got the
STAINLESS STEEL SLICE HOLD–DOWN FOR RC–26G CHAMBER
64-0253 (SHD-26GH/10)

It was definitely worth spending the money ($63, not much) to get a properly sized harp for the chamber I use R26-G. It fits perfectly and holds the slice down, and the 1 mm spacing is very adequate to advance a pipette to a spinal cord slice.