Hi Achamess, we are using a dye. we are just now trying to set up responses to K+ or capsaicin. K+ worked and we want to set up the imaging analysis. you have recently set up ca imaging in your lab? we arae using basic zeiss software for data acquisition and are now debating between imagej or cell profiler. we would prefer cell profiler, as it seems to be a very versatile system adn we want to set it up for other things as well... would appreciate any help you can offer. v.