Does anyone have a good solution for keeping a spinal cord slice (400 um) anchored in place in a perfusion chamber? When I was doing calcium imaging the other day with a slice, throughout the experiment the slice would move ever so slightly, presumably due tot he force of the perfusate. This messed up my imaging since the ROIs borders move off of the cells.
I was using a weighted U-shaped bar with a thin wire mesh band to hold the slice, which I understand is what people typically use for electrophysiology. Is there anything more robust though? What about coating a coverslip with poly-D-Lysine or something? Will that work?