Pax2 antibody help

[achamess]

@ShanTan

PAX2 Polyclonal Antibody (71-6000)

1:500.

This is the good one. Sorry you tried those other ones first.

You can see it here in one of my image panels:

Stuff I tried during grad school/post-doc that won't get published but could be useful

I talked about this in another thread http://forum.painresearcher.net/t/fosgfp-mice-for-noxious-stimulation-some-results/213 Here are some more images. Overall, this FosGFP line is good for activity-dependent labeling. It’s not perfect. There are some Fos+ (IHC) neurons that don’t get labeled, but overall, it’s useful. Could be good for electrophysiology. The stimulus here was 55ºC heat for 30s, followed by perfusion 2 hours after the stimulus. [fos-slices-panel-01-01]

Also, don’t fix too long. Light fixation. 1-2h post fix after your PFA perfusion.