Experimental Techniques


Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures: Protocols This category is for the sharing of experimental protocols. Animal Behavior and Models Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.) Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research.
About the Experimental Techniques category [Experimental Techniques] (2)
RNAscope (In situ hybridization) to study gene expression in pain-related tissues ( 2 ) [Experimental Techniques] (34)
WPI or Femtojet for Viral Injections? [Experimental Techniques] (4)
Astrocytes transduction in the spinal cord [Experimental Techniques] (2)
In Vivo Extracellular Recordings in Mouse Dorsal Horn - Anyone done it? [Electrophysiology] (3)
Iba-1 and GFAP antibodies [Reagents and Equipment] (13)
Pain Assessment Using the Rat and Mouse Formalin Tests [Animal Behavior and Models] (2)
Automated Video Analysis of Mouse Pain Behavior - Main Thread [Animal Behavior and Models] (2)
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord [Animal Behavior and Models] (1)
Looking for a good CGRP antibody (specific staining, low background) [Reagents and Equipment] (15)
Mouse Grimace Scale - Who's using it? [Animal Behavior and Models] (18)
Testing Dynamic Allodynia with Brush or Cotton - Tips and Tricks? [Animal Behavior and Models] (2)
Decerebrate mouse model for studies of the spinal cord circuits [Experimental Techniques] (1)
Detailed Protocol: Spared Nerve Injury Model of Neuropathic Pain in Mice [Animal Behavior and Models] (1)
Drop (immersion) fixation vs. transcardial perfusion of DRG and Spinal Cord [Experimental Techniques] (7)
How to embed spinal cord for cutting slices [Experimental Techniques] (3)
Mortar and pestle for grinding mouse DRG in liquid nitrogen? [Reagents and Equipment] (8)
Pseudorabies Virus (PRV) for DRG retrograde labeling? [Reagents and Equipment] (3)
Has anyone tried conditioned place aversion (CPA) with pain? [Animal Behavior and Models] (7)
Horizontal slices - Correctly identifying laminae [Experimental Techniques] (2)
BORIS v.6.1.2 is available [Experimental Techniques] (1)
Ipsilateral vs. Contralateral (Within-subject) vs. Sham/Vehicle Control for Unilateral Pain Models [Experimental Techniques] (4)
Good thread on what von Frey respond actually means [Animal Behavior and Models] (1)
Best marker for 'activated' microglia - CD68? [Microscopy (IHC/ISH/ICC/In Vivo)] (4)
Visualizing calcium transients in DRG axons using GCaMP [Experimental Techniques] (1)
Design and Construction of a Two-Temperature Preference Behavioral Assay for [Reagents and Equipment] (1)
Mechanical threshold testing after CFA in hindpaw -> Edema in mid hindpaw makes the mice less sensitive? [Animal Behavior and Models] (9)
Inhibitory Neurons in Lamina I-II [Microscopy (IHC/ISH/ICC/In Vivo)] (2)
Pax2 antibody help [Microscopy (IHC/ISH/ICC/In Vivo)] (7)
Sciatic Nerve Recordings - Tips and Tricks? [Experimental Techniques] (1)