Experimental Techniques


Protocols This category is for the sharing of experimental protocols.
Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.)
Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures:
Animal Behavior and Models
Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research.

About the Experimental Techniques category [Experimental Techniques] (2)
Mouse Grimace Scale - Who's using it? [Animal Behavior and Models] (18)
Testing Dynamic Allodynia with Brush or Cotton - Tips and Tricks? [Animal Behavior and Models] (2)
Decerebrate mouse model for studies of the spinal cord circuits [Experimental Techniques] (1)
Detailed Protocol: Spared Nerve Injury Model of Neuropathic Pain in Mice [Animal Behavior and Models] (1)
Drop (immersion) fixation vs. transcardial perfusion of DRG and Spinal Cord [Experimental Techniques] (7)
How to embed spinal cord for cutting slices [Experimental Techniques] (3)
Mortar and pestle for grinding mouse DRG in liquid nitrogen? [Reagents and Equipment] (8)
Pseudorabies Virus (PRV) for DRG retrograde labeling? [Reagents and Equipment] (3)
Has anyone tried conditioned place aversion (CPA) with pain? [Animal Behavior and Models] (7)
Horizontal slices - Correctly identifying laminae [Experimental Techniques] (2)
BORIS v.6.1.2 is available [Experimental Techniques] (1)
Ipsilateral vs. Contralateral (Within-subject) vs. Sham/Vehicle Control for Unilateral Pain Models [Experimental Techniques] (4)
Good thread on what von Frey respond actually means [Animal Behavior and Models] (1)
Best marker for 'activated' microglia - CD68? [Microscopy (IHC/ISH/ICC/In Vivo)] (4)
In Vivo Extracellular Recordings in Mouse Dorsal Horn - Anyone done it? [Electrophysiology] (1)
Visualizing calcium transients in DRG axons using GCaMP [Experimental Techniques] (1)
RNAscope (In situ hybridization) to study gene expression in pain-related tissues [Experimental Techniques] (18)
Design and Construction of a Two-Temperature Preference Behavioral Assay for [Reagents and Equipment] (1)
Mechanical threshold testing after CFA in hindpaw -> Edema in mid hindpaw makes the mice less sensitive? [Animal Behavior and Models] (9)
Inhibitory Neurons in Lamina I-II [Microscopy (IHC/ISH/ICC/In Vivo)] (2)
Pax2 antibody help [Microscopy (IHC/ISH/ICC/In Vivo)] (7)
Sciatic Nerve Recordings - Tips and Tricks? [Experimental Techniques] (1)
Iba-1 and GFAP antibodies [Reagents and Equipment] (9)
Electronic von Frey [Animal Behavior and Models] (6)
Sall1 as a microglia marker for IHC or FACS. Anyone tried? [Experimental Techniques] (2)
SOX9 as an Astrocyte Marker: Has anyone used in IHC or FACS? [Experimental Techniques] (1)
QuPath: A useful tool for analyzing microscopy images (tissue especially) [Microscopy (IHC/ISH/ICC/In Vivo)] (2)
I want to trace sensory fibers into the spinal cord. Do any retrograde tracers fill axons, not just cell bodies? [Experimental Techniques] (5)
Tissue clearing (CLARITY, CUBIC, iDisco...) on DRG and Spinal Cord? [Experimental Techniques] (2)