Experimental Techniques

Tester rating dependence in Mouse Behavior Assays (Up-down vs Frequency) [Experimental Techniques] (4)
Habituation Protocol for rodent Von Frey assay? [Animal Behavior and Models] (7)
Simplified Up-Down Method - (SUDO). Anyone use it? [Animal Behavior and Models] (7)
Pinprick Hyperalgesia - What stimulus? [Animal Behavior and Models] (2)
What rack and chambers do you use for Von Frey assay? [Animal Behavior and Models] (7)
White noise generator for Mouse behavior. A good idea? [Animal Behavior and Models] (1)
Inducible (Tamoxifen) Advillin-cre genotyping [Protocols] (4)
Cameras for video recording behavioral assays [Reagents and Equipment] (3)
Mouse Grimace Scale - Who's using it? [Animal Behavior and Models] (13)
Intrathecal siRNA injection for spinal neuron gene knockdown - Your experiences and tips? [Experimental Techniques] (8)
Ipsilateral vs Contralateral in imaging [Microscopy (IHC/ISH/ICC/In Vivo)] (4)
Identifying L4-L6 in Mouse spinal cord [Microscopy (IHC/ISH/ICC/In Vivo)] (2)
EF1a promoter to drive expression in DRG in vivo? [Reagents and Equipment] (2)
Colocalization analysis of cells - What's the best way? (ImageJ, Photoshop?) [Experimental Techniques] (4)
Video protocol of Spinal Nerve Transection in Mouse! [Experimental Techniques] (1)
Advillin-ChR2 -> Failure to thrive? [Animal Behavior and Models] (1)
Dorsal horn RNA? [Experimental Techniques] (14)
How do people accurately and methodically draw laminar boundaries in spinal cord IHC images? [Microscopy (IHC/ISH/ICC/In Vivo)] (9)
Tmem119 antibody for microglia [Reagents and Equipment] (3)
What software do you use to score behavior videos? [Animal Behavior and Models] (4)
Stuff I like - Lab Items about which I have an opinion [Reagents and Equipment] (4)
Keeping a spinal cord slice anchored/fixed in a perfusion chamber for calcium imaging [Protocols] (6)
Technique for making spinal cord slices (for electrophysiology)? [Experimental Techniques] (8)
Good qPCR primers for Nav1.8 and TRPV1 [Experimental Techniques] (5)
Resources for Microscopy Data Analysis [Microscopy (IHC/ISH/ICC/In Vivo)] (2)
Warming glass slides to prevent bubbles when thaw mounting? [Experimental Techniques] (2)
Best antibodies to label different neuronal populations [Microscopy (IHC/ISH/ICC/In Vivo)] (3)
DRG staining - samples fall of slides [Protocols] (9)
Drying slides before cover slipping [Protocols] (4)
Schwann cells protocol? [Experimental Techniques] (1)