# Uncategorized **URL:** https://forum.painresearcher.net/c/uncategorized/1.md [Latest](https://forum.painresearcher.net/latest.md) · [Categories](https://forum.painresearcher.net/categories.md) · [Tags](https://forum.painresearcher.net/tags.md) --- ## [Welcome to Pain Researcher a Production of the Pain Research Forum](https://forum.painresearcher.net/t/welcome-to-pain-researcher-a-production-of-the-pain-research-forum/8)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 1\ **Last updated:** [June 18, 2024, 10:10am UTC](https://forum.painresearcher.net/t/welcome-to-pain-researcher-a-production-of-the-pain-research-forum/8 "2024-06-18T10:10:26Z")
Welcome to Pain Researcher, a community forum for anyone involved or interested in the study of pain. The major purpose of this forum is to facilitate discussion around any and all topics related to the pain research. O… --- ## [Post-doc in pain researcher contributing to arthritis, cancer and stem cells](https://forum.painresearcher.net/t/post-doc-in-pain-researcher-contributing-to-arthritis-cancer-and-stem-cells/1475)
**Author:** [@ManishBais](https://forum.painresearcher.net/u/ManishBais)\ **Replies:** 0\ **Last updated:** [September 14, 2026, 12:18am UTC](https://forum.painresearcher.net/t/post-doc-in-pain-researcher-contributing-to-arthritis-cancer-and-stem-cells/1475 "2026-09-14T00:18:38Z")
We are interested in hiring an immediate pain and inflammation researcher to contribute to cross-project collaborations and translational research. Please see link below: Boston University hiring Postdoctoral Researcher… --- ## [Breaking Free from the "Bad Data" in Chronic Pain Research: Can Knowledge Graphs Help?](https://forum.painresearcher.net/t/breaking-free-from-the-bad-data-in-chronic-pain-research-can-knowledge-graphs-help/1325)
**Author:** [@bron\_free](https://forum.painresearcher.net/u/bron_free)\ **Replies:** 0\ **Last updated:** [January 23, 2025, 7:51pm UTC](https://forum.painresearcher.net/t/breaking-free-from-the-bad-data-in-chronic-pain-research-can-knowledge-graphs-help/1325 "2025-01-23T19:51:05Z")
The chronic pain research community is no stranger the challenges posed by poor-quality data, despite the substantial disease and societal burden it represents. Issues such as lack of provider knowledge, resistance to ex… --- ## [Antibodies for Human NaV1.7 and NaV1.8 antibodies that work well in Western Blotting](https://forum.painresearcher.net/t/antibodies-for-human-nav1-7-and-nav1-8-antibodies-that-work-well-in-western-blotting/1158)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 2\ **Last updated:** [June 14, 2024, 5:43pm UTC](https://forum.painresearcher.net/t/antibodies-for-human-nav1-7-and-nav1-8-antibodies-that-work-well-in-western-blotting/1158 "2024-06-14T17:43:40Z")
Any recommendations? @sshiers @mamocol --- ## [markers for synaptogenesis](https://forum.painresearcher.net/t/markers-for-synaptogenesis/1197)
**Author:** [@vijaykumarcbt](https://forum.painresearcher.net/u/vijaykumarcbt)\ **Replies:** 0\ **Last updated:** [May 2, 2024, 1:24pm UTC](https://forum.painresearcher.net/t/markers-for-synaptogenesis/1197 "2024-05-02T13:24:33Z")
Hello Everyone, What are the markers for synaptogenesis (the formation of new synapses) in the context of chronic pain? --- ## [50% threshold calculation](https://forum.painresearcher.net/t/50-threshold-calculation/1170)
**Author:** [@BishirM](https://forum.painresearcher.net/u/BishirM)\ **Replies:** 1\ **Last updated:** [January 24, 2024, 6:18pm UTC](https://forum.painresearcher.net/t/50-threshold-calculation/1170 "2024-01-24T18:18:09Z")
Hi, Can anyone please help me to understand the values in Von Frey 50% threshold calculation. I have difficulty in calculating the δ value. Is it the difference between two filaments applied? I am using the Ugo Basil v… --- ## [Primary mouse DRG immunocytochemistry troubleshooting - cell detachment](https://forum.painresearcher.net/t/primary-mouse-drg-immunocytochemistry-troubleshooting-cell-detachment/1126)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 5\ **Last updated:** [December 13, 2023, 7:31pm UTC](https://forum.painresearcher.net/t/primary-mouse-drg-immunocytochemistry-troubleshooting-cell-detachment/1126 "2023-12-13T19:31:44Z")
Another thread about ICC and cells not being adherent enough. We’ve recently had some issues with DRGs not making it through ICC. The edges have all the cells and center has mostly lost them. Here is an example from a r… --- ## [Von Frey Apparatus](https://forum.painresearcher.net/t/von-frey-apparatus/1114)
**Author:** [@BishirM](https://forum.painresearcher.net/u/BishirM)\ **Replies:** 6\ **Last updated:** [October 5, 2023, 6:41pm UTC](https://forum.painresearcher.net/t/von-frey-apparatus/1114 "2023-10-05T18:41:29Z")
Hi all, I am a graduate student who is new to pain research, and I am currently planning to use Von Frey Hairs for my upcoming study. I would greatly appreciate it if anyone could share a standard protocol or recommend … --- ## [cAMP signaling in dorsal root ganglion](https://forum.painresearcher.net/t/camp-signaling-in-dorsal-root-ganglion/1128)
**Author:** [@BishirM](https://forum.painresearcher.net/u/BishirM)\ **Replies:** 3\ **Last updated:** [August 25, 2023, 10:57pm UTC](https://forum.painresearcher.net/t/camp-signaling-in-dorsal-root-ganglion/1128 "2023-08-25T22:57:08Z")
Hi all, I would like to measure cAMP signaling in DRGs isolated from mice. Has anyone used fluorescence-based assays to measure cAMP in DRGs? Thank you in advance. Regards, Bishir. --- ## [Strong adherent cell lines that can withstand immunocytochemistry and that are good for heterologous expression of proteins?](https://forum.painresearcher.net/t/strong-adherent-cell-lines-that-can-withstand-immunocytochemistry-and-that-are-good-for-heterologous-expression-of-proteins/1123)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 6\ **Last updated:** [August 1, 2023, 10:33pm UTC](https://forum.painresearcher.net/t/strong-adherent-cell-lines-that-can-withstand-immunocytochemistry-and-that-are-good-for-heterologous-expression-of-proteins/1123 "2023-08-01T22:33:02Z")
I’ve been using HEK293 cells to express some plasmid constructs, and I’m frustrated by how easily HEK cells lift up from the surface, even when PDL-coated. Anyone have recommendations for more adherent cell lines that ca… --- ## [Systemic Administration of AAV PHP.S for Delivery to DRG](https://forum.painresearcher.net/t/systemic-administration-of-aav-php-s-for-delivery-to-drg/898)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 7\ **Last updated:** [April 3, 2023, 2:33pm UTC](https://forum.painresearcher.net/t/systemic-administration-of-aav-php-s-for-delivery-to-drg/898 "2023-04-03T14:33:22Z")
Has anyone used AAV-PHP.S in neonatal mice (P1) via the intraperitoneal route for delivery to DRG? In the original paper, they do IV admin and the transduction is great, but IP may be easier and it saves us some time wh… --- ## [GC-IB4 staining problems](https://forum.painresearcher.net/t/gc-ib4-staining-problems/311)
**Author:** [@Ewa-RUD](https://forum.painresearcher.net/u/Ewa-RUD)\ **Replies:** 14\ **Last updated:** [March 22, 2023, 11:05am UTC](https://forum.painresearcher.net/t/gc-ib4-staining-problems/311 "2023-03-22T11:05:37Z")
Hi everyone, I recently bought the fluorescently conjugated Isolectin GS-IB4 (Alexa Fluor 568). It doesn’t come with any information how to use it. Can you tell me how you guys dilute it to stain cells? Thanks in advance… --- ## [Lentiviral expression of sodium channels?](https://forum.painresearcher.net/t/lentiviral-expression-of-sodium-channels/1072)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 1\ **Last updated:** [March 4, 2023, 3:16am UTC](https://forum.painresearcher.net/t/lentiviral-expression-of-sodium-channels/1072 "2023-03-04T03:16:31Z")
Has anyone seen delivery of sodium channels (SCN9A, SCN10A, etc.) to primary cells (e.g. DRG culture) or cell lines via lentivirus? I haven’t come up with anything. I think the SCN genes, which are ~6K bases, might be to… --- ## [Nav1.8 expressing cell line](https://forum.painresearcher.net/t/nav1-8-expressing-cell-line/1001)
**Author:** [@mamocol](https://forum.painresearcher.net/u/mamocol)\ **Replies:** 2\ **Last updated:** [December 4, 2022, 2:24pm UTC](https://forum.painresearcher.net/t/nav1-8-expressing-cell-line/1001 "2022-12-04T14:24:01Z")
Hi everyone, Does anyone know of a human cell-line that highly expresses Nav1.8? Our current only (very expensive) option is human DRG, as all cell lines we tested have very low expression levels. Thank you! --- ## [TRPV1 Neuromab (N221/17)](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 4\ **Last updated:** [October 29, 2022, 9:48pm UTC](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966 "2022-10-29T21:48:06Z")
@sshiers Do you have thoughts on this TRPV1 antibody? Does it work for mouse and human? --- ## [Distinct neural networks derived from galanin-containing nociceptors and neurotensin-expressing pruriceptors](https://forum.painresearcher.net/t/distinct-neural-networks-derived-from-galanin-containing-nociceptors-and-neurotensin-expressing-pruriceptors/1027)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [October 27, 2022, 5:22pm UTC](https://forum.painresearcher.net/t/distinct-neural-networks-derived-from-galanin-containing-nociceptors-and-neurotensin-expressing-pruriceptors/1027 "2022-10-27T17:22:47Z")
https://www.pnas.org/doi/10.1073/pnas.2118501119 Nice work. They figured out how to get HSV129 to work in mouse DRGs --- ## [Antibodies for nociceptors that work well in skin?](https://forum.painresearcher.net/t/antibodies-for-nociceptors-that-work-well-in-skin/1020)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 5\ **Last updated:** [October 1, 2022, 3:15pm UTC](https://forum.painresearcher.net/t/antibodies-for-nociceptors-that-work-well-in-skin/1020 "2022-10-01T15:15:32Z")
We’re doing some skin staining with PGP9.5. Works great but I want to look for something more specific for C- and/or Ad-fibers. Can anyone recommend antibodies that work for targets like TRPV1, CGRP, Substance P, Nav1.7/… --- ## [Pain is Not a “thing”: How That Error Affects Language and Logic in Pain](https://forum.painresearcher.net/t/pain-is-not-a-thing-how-that-error-affects-language-and-logic-in-pain/1006)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [August 7, 2022, 7:25pm UTC](https://forum.painresearcher.net/t/pain-is-not-a-thing-how-that-error-affects-language-and-logic-in-pain/1006 "2022-08-07T19:25:44Z")
Interesting discussion of common terminology and their meaning in pain medicine --- ## [Protocol for calcium imaging of mouse DRG neurons](https://forum.painresearcher.net/t/protocol-for-calcium-imaging-of-mouse-drg-neurons/992)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 2\ **Last updated:** [June 22, 2022, 8:55pm UTC](https://forum.painresearcher.net/t/protocol-for-calcium-imaging-of-mouse-drg-neurons/992 "2022-06-22T20:55:31Z")
Hi all. Wanting to do some calcium imaging, either with Fura-2 or GCaMP on dissociated DRGs. Does anyone have a detailed protocol they use? We have one, but I’m wanting to just cross-reference with others to optimize. @t… --- ## [Tail pinch assay - Mechanical](https://forum.painresearcher.net/t/tail-pinch-assay-mechanical/980)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 4\ **Last updated:** [May 19, 2022, 11:33am UTC](https://forum.painresearcher.net/t/tail-pinch-assay-mechanical/980 "2022-05-19T11:33:17Z")
Has anyone used the Rodent Pincher Analgesia assay for mechanical nociception? @tberta @thicunha @tonellor @ShanTan @CandlerPaige https://www.iitcinc.com/rodentpincher.html It seems fairly straightforward. We’ve been … --- ## [Question about GFAP](https://forum.painresearcher.net/t/question-about-gfap/922)
**Author:** [@sshiers](https://forum.painresearcher.net/u/sshiers)\ **Replies:** 16\ **Last updated:** [March 14, 2022, 3:35pm UTC](https://forum.painresearcher.net/t/question-about-gfap/922 "2022-03-14T15:35:20Z")
I have seen papers using GFAP staining to assess satellite glial cell activation (gliosis) - my question is: is GFAP only expressed during SGC activation, or is it expressed at basal levels and then there is an increase … --- ## [Knee Injections in Mouse](https://forum.painresearcher.net/t/knee-injections-in-mouse/928)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [February 8, 2022, 4:02pm UTC](https://forum.painresearcher.net/t/knee-injections-in-mouse/928 "2022-02-08T16:02:56Z")
Does anyone have a protocol or video they’re willing to share for injections into the knee of mouse? I need it to be precise without leakage. Will be dye or virus. @thicunha @tberta @tonellor @dmolliver Thanks! --- ## [Proportion and number of DRG neurons innervating skin and other targets](https://forum.painresearcher.net/t/proportion-and-number-of-drg-neurons-innervating-skin-and-other-targets/906)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [November 10, 2021, 10:36pm UTC](https://forum.painresearcher.net/t/proportion-and-number-of-drg-neurons-innervating-skin-and-other-targets/906 "2021-11-10T22:36:47Z")
Does anyone have a reference showing the proportion of #DRG neurons that are cutaneous at different spinal levels? @dmolliver @liz @tberta --- ## [Longitudinal intravital imaging nerve degeneration and sprouting in the toes](https://forum.painresearcher.net/t/longitudinal-intravital-imaging-nerve-degeneration-and-sprouting-in-the-toes/846)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 1\ **Last updated:** [June 15, 2021, 5:16am UTC](https://forum.painresearcher.net/t/longitudinal-intravital-imaging-nerve-degeneration-and-sprouting-in-the-toes/846 "2021-06-15T05:16:02Z")
--- ## [Which non-traumatic neuropathy assay most reliably produces pain-like behaviors in your hands?](https://forum.painresearcher.net/t/which-non-traumatic-neuropathy-assay-most-reliably-produces-pain-like-behaviors-in-your-hands/823)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 8\ **Last updated:** [March 31, 2021, 12:38pm UTC](https://forum.painresearcher.net/t/which-non-traumatic-neuropathy-assay-most-reliably-produces-pain-like-behaviors-in-your-hands/823 "2021-03-31T12:38:54Z")
Pain research relies heavily on traumatic models of nerve injury such as SNI. While these model well some aspects of neuropathic pain, the majority of neuropathy seen in the clinic is non-traumatic (e.g. diabetic, chemot… --- ## [Adult DRG explants or dissociated cultures for axonal degeneration studies?](https://forum.painresearcher.net/t/adult-drg-explants-or-dissociated-cultures-for-axonal-degeneration-studies/751)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 8\ **Last updated:** [March 26, 2021, 6:02pm UTC](https://forum.painresearcher.net/t/adult-drg-explants-or-dissociated-cultures-for-axonal-degeneration-studies/751 "2021-03-26T18:02:14Z")
Does anyone have any experience using adult DRGs, either as explants or dissociated cultures, for axonal degeneration studies? My preference is to use adult because the process/disease I want to study occurs in adults, a… --- ## [TMEM119 Cre ERT2](https://forum.painresearcher.net/t/tmem119-cre-ert2/787)
**Author:** [@ShanTan](https://forum.painresearcher.net/u/ShanTan)\ **Replies:** 1\ **Last updated:** [January 26, 2021, 3:21pm UTC](https://forum.painresearcher.net/t/tmem119-cre-ert2/787 "2021-01-26T15:21:10Z")
Hi guys, Has anyone crossed the TMEM119 Cre ERT2 line with Rosa IDTR line to try to deplete microglia? Similar to the CX3CR1 line but should be more specific. We’ve done so with some success, but one of our collaborator… --- ## [A great week for pain/somatosensory research - 2020-11-14](https://forum.painresearcher.net/t/a-great-week-for-pain-somatosensory-research-2020-11-14/775)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [November 14, 2020, 2:00pm UTC](https://forum.painresearcher.net/t/a-great-week-for-pain-somatosensory-research-2020-11-14/775 "2020-11-14T14:00:41Z")
3/4 articles on that banner at Neuron are about pain/somatosensation It’s an exciting time to be in pain neuroscience for sure --- ## [Mechanical Allodynia Circuitry in the Dorsal Horn Is Defined by the Nature of the Injury](https://forum.painresearcher.net/t/mechanical-allodynia-circuitry-in-the-dorsal-horn-is-defined-by-the-nature-of-the-injury/774)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [November 14, 2020, 1:59pm UTC](https://forum.painresearcher.net/t/mechanical-allodynia-circuitry-in-the-dorsal-horn-is-defined-by-the-nature-of-the-injury/774 "2020-11-14T13:59:04Z")
https://www.cell.com/neuron/fulltext/S0896-6273(20)30823-0 Congrats @RPSeal @cedric.peirs and team --- ## [Culturing TG neurons](https://forum.painresearcher.net/t/culturing-tg-neurons/707)
**Author:** [@DaraBree](https://forum.painresearcher.net/u/DaraBree)\ **Replies:** 6\ **Last updated:** [March 5, 2020, 5:55pm UTC](https://forum.painresearcher.net/t/culturing-tg-neurons/707 "2020-03-05T17:55:55Z")
We culture DRG neurons and have become fairly consistent at it but are continuously looking to increase the yield of neurons we get. Would adding trigeminal ganglion neurons into the mix be one way of doing this? From… [Next page](https://forum.painresearcher.net/c/uncategorized/1.md?page=1)