# Latest **URL:** https://forum.painresearcher.net/latest.md [Latest](https://forum.painresearcher.net/latest.md) · [Categories](https://forum.painresearcher.net/categories.md) · [Tags](https://forum.painresearcher.net/tags.md) --- ## [Welcome to Pain Researcher a Production of the Pain Research Forum](https://forum.painresearcher.net/t/welcome-to-pain-researcher-a-production-of-the-pain-research-forum/8)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 1\ **Last updated:** [June 18, 2024, 10:10am UTC](https://forum.painresearcher.net/t/welcome-to-pain-researcher-a-production-of-the-pain-research-forum/8 "2024-06-18T10:10:26Z")
Welcome to Pain Researcher, a community forum for anyone involved or interested in the study of pain. The major purpose of this forum is to facilitate discussion around any and all topics related to the pain research. O… --- ## [Probelm with Patch clamp of Dissociated DRG neurons](https://forum.painresearcher.net/t/probelm-with-patch-clamp-of-dissociated-drg-neurons/822)
**Author:** [@Abderaouf](https://forum.painresearcher.net/u/Abderaouf)\ **Replies:** 7\ **Last updated:** [September 17, 2026, 6:50pm UTC](https://forum.painresearcher.net/t/probelm-with-patch-clamp-of-dissociated-drg-neurons/822 "2026-09-17T18:50:14Z")
Hi everyone We are trying to study the electrophysiological parameters in dissociated DRG primary culture cells, we have been trying for a while now to get a whole cell configuration to do a patch clamp experiments but … --- ## [Post-doc in pain researcher contributing to arthritis, cancer and stem cells](https://forum.painresearcher.net/t/post-doc-in-pain-researcher-contributing-to-arthritis-cancer-and-stem-cells/1475)
**Author:** [@ManishBais](https://forum.painresearcher.net/u/ManishBais)\ **Replies:** 0\ **Last updated:** [September 14, 2026, 12:18am UTC](https://forum.painresearcher.net/t/post-doc-in-pain-researcher-contributing-to-arthritis-cancer-and-stem-cells/1475 "2026-09-14T00:18:38Z")
We are interested in hiring an immediate pain and inflammation researcher to contribute to cross-project collaborations and translational research. Please see link below: Boston University hiring Postdoctoral Researcher… --- ## [Call for Applications: HEAL K12 Clinical Pain Career Development Award](https://forum.painresearcher.net/t/call-for-applications-heal-k12-clinical-pain-career-development-award/1474)
**Author:** [@saraske](https://forum.painresearcher.net/u/saraske)\ **Replies:** 0\ **Last updated:** [September 1, 2026, 12:21pm UTC](https://forum.painresearcher.net/t/call-for-applications-heal-k12-clinical-pain-career-development-award/1474 "2026-09-01T12:21:18Z")
The Chronic Pain and Fatigue Research Center at the University of Michigan is requesting applications for the HEAL\* National K12 Clinical Pain Career Development Award (HEAL K12). https://heal-k12.med.umich.edu/ The H… --- ## [Postdoctoral Research Fellow - Browne Lab at University College London](https://forum.painresearcher.net/t/postdoctoral-research-fellow-browne-lab-at-university-college-london/1470)
**Author:** [@lebrowne](https://forum.painresearcher.net/u/lebrowne)\ **Replies:** 0\ **Last updated:** [July 7, 2026, 3:02pm UTC](https://forum.painresearcher.net/t/postdoctoral-research-fellow-browne-lab-at-university-college-london/1470 "2026-07-07T15:02:23Z")
We are seeking a motivated, creative and technically strong Postdoctoral Research Fellow to develop preclinical neurotechnology for adaptive neural interfaces. The project will focus on building closed-loop experimental… --- ## [Post-doctoral Research Position in Weir lab- Spinal Cord Group, University of Glasgow](https://forum.painresearcher.net/t/post-doctoral-research-position-in-weir-lab-spinal-cord-group-university-of-glasgow/1469)
**Author:** [@GregW](https://forum.painresearcher.net/u/GregW)\ **Replies:** 0\ **Last updated:** [June 16, 2026, 8:16pm UTC](https://forum.painresearcher.net/t/post-doctoral-research-position-in-weir-lab-spinal-cord-group-university-of-glasgow/1469 "2026-06-16T20:16:50Z")
We have an exciting opportunity for a Research Associate to make a leading contribution to a project entitled “Targeting the primary afferent drivers of neuropathic pain,” working with Dr Greg Weir and his neurobiology o… --- ## [Alternative to TUBB3 (TUJ1) for axon staining, non-neuronal staining](https://forum.painresearcher.net/t/alternative-to-tubb3-tuj1-for-axon-staining-non-neuronal-staining/844)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 2\ **Last updated:** [April 13, 2026, 3:27pm UTC](https://forum.painresearcher.net/t/alternative-to-tubb3-tuj1-for-axon-staining-non-neuronal-staining/844 "2026-04-13T15:27:25Z")
Hi all, I’m looking at axons from mouse and human DRG in vitro. The standard stain is TUBB3. I’m using the Tuj-1 clone from Biolegend (formerly Covaris), which is the gold-standard as far as I can tell. https://www.bio… --- ## [Immunostaining of paraffin-embedded tissues with bone](https://forum.painresearcher.net/t/immunostaining-of-paraffin-embedded-tissues-with-bone/1453)
**Author:** [@wtheodoro](https://forum.painresearcher.net/u/wtheodoro)\ **Replies:** 2\ **Last updated:** [November 4, 2025, 2:05pm UTC](https://forum.painresearcher.net/t/immunostaining-of-paraffin-embedded-tissues-with-bone/1453 "2025-11-04T14:05:39Z")
Hello everyone, I’ve been encountering high background when immunostaining paraffin-embedded, fixed tissues that include both soft and bony components. These specimens are periapical tissues obtained from human teeth d… --- ## [RNAscope (In situ hybridization) to study gene expression in pain-related tissues](https://forum.painresearcher.net/t/rnascope-in-situ-hybridization-to-study-gene-expression-in-pain-related-tissues/102)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 88\ **Last updated:** [October 23, 2025, 2:43pm UTC](https://forum.painresearcher.net/t/rnascope-in-situ-hybridization-to-study-gene-expression-in-pain-related-tissues/102 "2025-10-23T14:43:54Z")
In situ hybridization is a powerful method to interrogate gene expression in tissues. It is known as being a challenging procedure due to the many steps where errors and obstacles arise. We've recently begun to use a com… --- ## [Pain behaviors in face](https://forum.painresearcher.net/t/pain-behaviors-in-face/1451)
**Author:** [@Malaika](https://forum.painresearcher.net/u/Malaika)\ **Replies:** 0\ **Last updated:** [October 15, 2025, 6:44pm UTC](https://forum.painresearcher.net/t/pain-behaviors-in-face/1451 "2025-10-15T18:44:57Z")
Does anyone have any recommendations on what good programs there are to track facial pain behaviors specifically things like whisking, face wiping, grimacing, etc? I know Pain Face is one that many use but it seems to no… --- ## [Tone-pain conditioning](https://forum.painresearcher.net/t/tone-pain-conditioning/1443)
**Author:** [@Malaika](https://forum.painresearcher.net/u/Malaika)\ **Replies:** 2\ **Last updated:** [September 25, 2025, 6:09pm UTC](https://forum.painresearcher.net/t/tone-pain-conditioning/1443 "2025-09-25T18:09:59Z")
Has anyone done a tone-pain conditioning task specifically using a laser to administer pain in mice? I’m curious to know the parameters (i.e. how long tone was played for, what intensity of the laser was used, what kind … --- ## [Human on Human high IgG background on human DRG/nerve](https://forum.painresearcher.net/t/human-on-human-high-igg-background-on-human-drg-nerve/784)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 4\ **Last updated:** [September 5, 2025, 12:19pm UTC](https://forum.painresearcher.net/t/human-on-human-high-igg-background-on-human-drg-nerve/784 "2025-09-05T12:19:00Z")
I’m attempting IHC on human DRG/nerve using human serum (containing putative anti-human antibodies). The issue I’m running into is that there is huge background with just the secondary alone (goat anti-human IgG). How … --- ## [Discussion about how to assess pain and stress in extremely preterm infants (\<26 weeks) using behavioral scales and pattern recognition](https://forum.painresearcher.net/t/discussion-about-how-to-assess-pain-and-stress-in-extremely-preterm-infants-26-weeks-using-behavioral-scales-and-pattern-recognition/1442)
**Author:** [@Erik](https://forum.painresearcher.net/u/Erik)\ **Replies:** 0\ **Last updated:** [August 22, 2025, 6:59pm UTC](https://forum.painresearcher.net/t/discussion-about-how-to-assess-pain-and-stress-in-extremely-preterm-infants-26-weeks-using-behavioral-scales-and-pattern-recognition/1442 "2025-08-22T18:59:33Z")
Hi everyone, My name is Erik Koning and I’m currently working on a PhD project focused on pain assessment in extremely preterm infants, specifically those born before 26 weeks of gestation. While several behavioral pain… --- ## [EF1a promoter to drive expression in DRG in vivo?](https://forum.painresearcher.net/t/ef1a-promoter-to-drive-expression-in-drg-in-vivo/331)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 14\ **Last updated:** [August 19, 2025, 3:56pm UTC](https://forum.painresearcher.net/t/ef1a-promoter-to-drive-expression-in-drg-in-vivo/331 "2025-08-19T15:56:49Z")
Has anyone used EF1a promoters to drive transgene expression in DRGs in vivo? I’ve had success with hSyn, and recently, a paper from Haenraets et al showed CAG works well for DRG. But EF1a is also very popular and it’d b… --- ## [Inquiry on NR2B Primary Antibody for Mouse Fluorescent Immunostaining](https://forum.painresearcher.net/t/inquiry-on-nr2b-primary-antibody-for-mouse-fluorescent-immunostaining/1432)
**Author:** [@ZoeChen](https://forum.painresearcher.net/u/ZoeChen)\ **Replies:** 1\ **Last updated:** [August 14, 2025, 3:07pm UTC](https://forum.painresearcher.net/t/inquiry-on-nr2b-primary-antibody-for-mouse-fluorescent-immunostaining/1432 "2025-08-14T15:07:25Z")
Dear all, I am a graduate student currently working on fluorescent immunostaining of mice spinal cord tissue. I am searching for a primary antibody against NR2B that is suitable for immunofluorescence and raised in a ho… --- ## [Transgenic Cre mouse for labeling periphery nerves](https://forum.painresearcher.net/t/transgenic-cre-mouse-for-labeling-periphery-nerves/1398)
**Author:** [@Jesicawiiiij](https://forum.painresearcher.net/u/Jesicawiiiij)\ **Replies:** 2\ **Last updated:** [August 11, 2025, 2:59pm UTC](https://forum.painresearcher.net/t/transgenic-cre-mouse-for-labeling-periphery-nerves/1398 "2025-08-11T14:59:07Z")
Hi everyone! I’m looking for Cre mouse line recommendations\*\* for comprehensive peripheral nerve labeling. My goal is to cross Cre mice with fluorescent reporters, then perform tissue clearing and whole-body peripheral n… --- ## [Request to provide a standard method of Mouse DRG culture and Immunocytochemistry Protocol](https://forum.painresearcher.net/t/request-to-provide-a-standard-method-of-mouse-drg-culture-and-immunocytochemistry-protocol/1415)
**Author:** [@Hemanta](https://forum.painresearcher.net/u/Hemanta)\ **Replies:** 0\ **Last updated:** [July 27, 2025, 6:37am UTC](https://forum.painresearcher.net/t/request-to-provide-a-standard-method-of-mouse-drg-culture-and-immunocytochemistry-protocol/1415 "2025-07-27T06:37:28Z")
I am searching for DRG culture and ICC protocol. --- ## [New Pain Research Forum Website Official Launch Date July 15th 2025](https://forum.painresearcher.net/t/new-pain-research-forum-website-official-launch-date-july-15th-2025/1365)
**Author:** [@PRF-Editorial-Board](https://forum.painresearcher.net/u/PRF-Editorial-Board)\ **Replies:** 4\ **Last updated:** [July 15, 2025, 10:15pm UTC](https://forum.painresearcher.net/t/new-pain-research-forum-website-official-launch-date-july-15th-2025/1365 "2025-07-15T22:15:23Z")
Exciting news! The Pain Research Forum will have a new user-friendly, aesthetically pleasing website with an official launch date of July 15th, 2025. It will be linked to the IASP website and is free to use. Registrat… --- ## [Housekeeping proteins for western blot that do not change following nerve injury](https://forum.painresearcher.net/t/housekeeping-proteins-for-western-blot-that-do-not-change-following-nerve-injury/1352)
**Author:** [@gg9761](https://forum.painresearcher.net/u/gg9761)\ **Replies:** 3\ **Last updated:** [July 9, 2025, 1:53pm UTC](https://forum.painresearcher.net/t/housekeeping-proteins-for-western-blot-that-do-not-change-following-nerve-injury/1352 "2025-07-09T13:53:29Z")
Hi all, I need to run a western blot on protein lysate extracted from mouse DRGs. One of my groups has undergone a spared nerve injury and I’m aware that certain commonly used housekeeping proteins can be up or downregu… --- ## [Ideal age to record PSCs from mice spinal cord without stimulation electrode](https://forum.painresearcher.net/t/ideal-age-to-record-pscs-from-mice-spinal-cord-without-stimulation-electrode/1359)
**Author:** [@anutoshroy](https://forum.painresearcher.net/u/anutoshroy)\ **Replies:** 2\ **Last updated:** [April 16, 2025, 4:10pm UTC](https://forum.painresearcher.net/t/ideal-age-to-record-pscs-from-mice-spinal-cord-without-stimulation-electrode/1359 "2025-04-16T16:10:32Z")
Hello! I have two queries regarding ephys from spinal cord slice, For recording of Post synaptic currents, is there any method other than stimulation electrode to record the PSCs in mice spinal cord in P0-P12 mice spi… --- ## [DAB staining Immunohistochemistery](https://forum.painresearcher.net/t/dab-staining-immunohistochemistery/1353)
**Author:** [@HebaReda](https://forum.painresearcher.net/u/HebaReda)\ **Replies:** 2\ **Last updated:** [April 6, 2025, 9:20pm UTC](https://forum.painresearcher.net/t/dab-staining-immunohistochemistery/1353 "2025-04-06T21:20:39Z")
Hi, I am planning to use DAB with nickel in immunohistochemistery so what is the best concentration that should be used please as I am confused with that? I was preparing it freshly by adding 2.7 mg in 10 ml PBS (so a … --- ## [Frozen vs. Non-frozen IHC (Fixed) Spinal Cords. Thoughts?](https://forum.painresearcher.net/t/frozen-vs-non-frozen-ihc-fixed-spinal-cords-thoughts/202)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 13\ **Last updated:** [March 17, 2025, 8:26pm UTC](https://forum.painresearcher.net/t/frozen-vs-non-frozen-ihc-fixed-spinal-cords-thoughts/202 "2025-03-17T20:26:46Z")
Has anyone done free-floating spinal cord sectioning/staining using fixed, but not frozen tissue using a vibratome? For example, I noticed Andrew Todd does much of his IHC this way, and the images look great. Any thought… --- ## [Welcome to The Pain Researcher a Production of the Pain Research Forum](https://forum.painresearcher.net/t/welcome-to-the-pain-researcher-a-production-of-the-pain-research-forum/1348)
**Author:** [@RPSeal](https://forum.painresearcher.net/u/RPSeal)\ **Replies:** 0\ **Last updated:** [March 17, 2025, 8:26pm UTC](https://forum.painresearcher.net/t/welcome-to-the-pain-researcher-a-production-of-the-pain-research-forum/1348 "2025-03-17T20:26:00Z")
This is a community forum for anyone involved or interested in the study of pain. The forum exists to discuss all topics related to pain research and particularly the sharing of knowledge needed to properly execute pa… --- ## [Pain Research Fellow - Koch Lab @ University College London](https://forum.painresearcher.net/t/pain-research-fellow-koch-lab-university-college-london/1346)
**Author:** [@sckoch](https://forum.painresearcher.net/u/sckoch)\ **Replies:** 1\ **Last updated:** [March 16, 2025, 12:33pm UTC](https://forum.painresearcher.net/t/pain-research-fellow-koch-lab-university-college-london/1346 "2025-03-16T12:33:46Z")
This is an exciting opportunity to join the Koch lab within the Medawar Pain and Somatosensory Group. We investigate adaptive plasticity in sensorimotor circuits. As a Research Fellow in our group, you will lead a proje… --- ## [Structure-guided design of a peripherally restricted chemogenetic system](https://forum.painresearcher.net/t/structure-guided-design-of-a-peripherally-restricted-chemogenetic-system/1328)
**Author:** [@vhms0706](https://forum.painresearcher.net/u/vhms0706)\ **Replies:** 3\ **Last updated:** [February 24, 2025, 12:15pm UTC](https://forum.painresearcher.net/t/structure-guided-design-of-a-peripherally-restricted-chemogenetic-system/1328 "2025-02-24T12:15:14Z")
Recent paper from Dr. Bryan Roth Lab on developing peripherally restricting DREADD system using receptor engineering: Structure-guided design of a peripherally restricted chemogenetic system - ScienceDirect I have a rea… --- ## [Postdoctoral Research Associate – Chamessian Lab @ WashU - Targeted Protein Degradation of Ion Channels for Pain Therapeutic Development](https://forum.painresearcher.net/t/postdoctoral-research-associate-chamessian-lab-washu-targeted-protein-degradation-of-ion-channels-for-pain-therapeutic-development/1336)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [February 22, 2025, 4:43pm UTC](https://forum.painresearcher.net/t/postdoctoral-research-associate-chamessian-lab-washu-targeted-protein-degradation-of-ion-channels-for-pain-therapeutic-development/1336 "2025-02-22T16:43:01Z")
Link to preprint describing the foundations of this project: https://www.biorxiv.org/content/10.1101/2025.01.21.634079v1 My lab is looking for qualified candidates to lead this exciting and ambitious project. Please … --- ## [Breaking Free from the "Bad Data" in Chronic Pain Research: Can Knowledge Graphs Help?](https://forum.painresearcher.net/t/breaking-free-from-the-bad-data-in-chronic-pain-research-can-knowledge-graphs-help/1325)
**Author:** [@bron\_free](https://forum.painresearcher.net/u/bron_free)\ **Replies:** 0\ **Last updated:** [January 23, 2025, 7:51pm UTC](https://forum.painresearcher.net/t/breaking-free-from-the-bad-data-in-chronic-pain-research-can-knowledge-graphs-help/1325 "2025-01-23T19:51:05Z")
The chronic pain research community is no stranger the challenges posed by poor-quality data, despite the substantial disease and societal burden it represents. Issues such as lack of provider knowledge, resistance to ex… --- ## [Spiders, Snow, and Perceptions: How Virtual Reality Is Evolving in Acute and Chronic Pain Management](https://forum.painresearcher.net/t/spiders-snow-and-perceptions-how-virtual-reality-is-evolving-in-acute-and-chronic-pain-management/1313)
**Author:** [@PRF-Editorial-Board](https://forum.painresearcher.net/u/PRF-Editorial-Board)\ **Replies:** 0\ **Last updated:** [December 6, 2024, 6:54pm UTC](https://forum.painresearcher.net/t/spiders-snow-and-perceptions-how-virtual-reality-is-evolving-in-acute-and-chronic-pain-management/1313 "2024-12-06T18:54:03Z")
Check out PRF’s latest News article from Lincoln Tracy about the use of virtual reality (VR) in pain management, including a retrospective look on one of the first pain research studies to use VR and some words from the … --- ## [Establishing a Role for the Rostral Ventral Medulla in Morphine Analgesia](https://forum.painresearcher.net/t/establishing-a-role-for-the-rostral-ventral-medulla-in-morphine-analgesia/1311)
**Author:** [@PRF-Editorial-Board](https://forum.painresearcher.net/u/PRF-Editorial-Board)\ **Replies:** 0\ **Last updated:** [November 8, 2024, 8:36pm UTC](https://forum.painresearcher.net/t/establishing-a-role-for-the-rostral-ventral-medulla-in-morphine-analgesia/1311 "2024-11-08T20:36:16Z")
A new study identifies key cells in an ensemble of morphine-responsive neurons within the RVM that promote mechanical antinociception. Tell us what you think of these results and how they might affect your research! --- ## [Gene therapy for chronic pain management](https://forum.painresearcher.net/t/gene-therapy-for-chronic-pain-management/1299)
**Author:** [@PRF-Editorial-Board](https://forum.painresearcher.net/u/PRF-Editorial-Board)\ **Replies:** 0\ **Last updated:** [October 11, 2024, 7:18pm UTC](https://forum.painresearcher.net/t/gene-therapy-for-chronic-pain-management/1299 "2024-10-11T19:18:10Z")
Check out this newly published comprehensive review on gene therapy approaches being developed for the treatment of chronic pain. [Next page](https://forum.painresearcher.net/latest.md?page=1)