# Best storage method for peripheral nerves?

**URL:** <https://forum.painresearcher.net/t/best-storage-method-for-peripheral-nerves/1078>\
**Category:** Protocols\
**Created:** [March 12, 2023, 2:50pm UTC](https://forum.painresearcher.net/t/best-storage-method-for-peripheral-nerves/1078 "2023-03-12T14:50:52Z")\
**Posts on this page:** 3\
**Page:** 1

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**Author:** ![Zara109](https://avatars.discourse-cdn.com/v4/letter/z/e36b37/32.png) [@Zara109](https://forum.painresearcher.net/u/Zara109)\
**Post date:** [March 12, 2023, 2:50pm UTC](https://forum.painresearcher.net/t/best-storage-method-for-peripheral-nerves/1078/1 "2023-03-12T14:50:52Z")

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Hi all! I need to keep some rat sciatic nerves in storage for a couple months and I’m wondering what’s the best way of doing that, as I’m new to this. Some have suggested using cryoprotectants like glycerol-dmso and storage at -80C. Any suggestions are welcome! Thanks.

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [March 15, 2023, 8:05pm UTC](https://forum.painresearcher.net/t/best-storage-method-for-peripheral-nerves/1078/2 "2023-03-15T20:05:20Z")

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Can you provide more detail about how the tissues were preserved? Are they fresh? PFA-fixed? What are your end applications?

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**Author:** ![Anji](https://avatars.discourse-cdn.com/v4/letter/a/bbce88/32.png) [@Anji](https://forum.painresearcher.net/u/Anji)\
**Post date:** [May 15, 2023, 3:55am UTC](https://forum.painresearcher.net/t/best-storage-method-for-peripheral-nerves/1078/3 "2023-05-15T03:55:06Z")

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Have worked with sciatic nerves in the past. Would be great if you can share what experiments you are planning to do from the stored nerves??