# Best "supraspinal" nociceptive assays?

**URL:** <https://forum.painresearcher.net/t/best-supraspinal-nociceptive-assays/656>\
**Category:** Animal Behavior and Models\
**Created:** [September 9, 2019, 3:41pm UTC](https://forum.painresearcher.net/t/best-supraspinal-nociceptive-assays/656 "2019-09-09T15:41:57Z")\
**Posts on this page:** 1\
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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [September 10, 2019, 12:23am UTC](https://forum.painresearcher.net/t/best-supraspinal-nociceptive-assays/656/2 "2019-09-10T00:23:03Z")

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@ShanTan  
See this thread:

> [@Has anyone tried conditioned place aversion (CPA) with pain?](https://forum.painresearcher.net/t/has-anyone-tried-conditioned-place-aversion-cpa-with-pain/347):
>
> I saw this CPA assay in a paper from Cheng et al. (2017) [https://www.nature.com/articles/nn.4549](https://www.nature.com/articles/nn.4549) [07 PM] This looks laborious but useful. To measure the negative valence effect associated with brush-evoked dynamic mechanical hypersensitivity, we used a biased compartment-assignment proce- dure, in which we measured the influence of VT3Lbx1 neurons on the time of mice spending in the dark compartment receiving conditional stimulations. The CPA apparatus consisted of two chambers (10 × 10 × 1…

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