# FACS neurons and astrocytes **URL:** \ **Category:** Experimental Techniques\ **Created:** [August 11, 2021, 3:32am UTC](https://forum.painresearcher.net/t/facs-neurons-and-astrocytes/877 "2021-08-11T03:32:48Z")\ **Posts on this page:** 6\ **Page:** 1
**Author:** ![ShanTan](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/shantan/32/242_2.png) [@ShanTan](https://forum.painresearcher.net/u/ShanTan)\ **Post date:** [August 11, 2021, 3:32am UTC](https://forum.painresearcher.net/t/facs-neurons-and-astrocytes/877/1 "2021-08-11T03:32:48Z")
Has anyone had any success sorting neurons and astrocytes? Preferably not with transgenic lines, but rather with antibodies. Any help is appreciated. ---
**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\ **Post date:** [August 12, 2021, 12:29pm UTC](https://forum.painresearcher.net/t/facs-neurons-and-astrocytes/877/2 "2021-08-12T12:29:00Z")
Hi @ShanTan we have established sorting of astrocytes in the spinal cord of mice using anti-ACSA2 antibody anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250). Neurons are more complicated due to viability. You can find more information in our preprint paper ([https://www.biorxiv.org/content/10.1101/2021.01.25.428142v2](https://www.biorxiv.org/content/10.1101/2021.01.25.428142v2)). ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [August 22, 2021, 2:26pm UTC](https://forum.painresearcher.net/t/facs-neurons-and-astrocytes/877/3 "2021-08-22T14:26:43Z")
Where are you dissociating neurons from - brain or spinal cord? Adult spinal cord neurons are very hard to dissociate and keep alive. You could try the following: > **[Anti Rat CD90 Antibody, clone OX-7 | Bio-Rad](https://www.bio-rad-antibodies.com/monoclonal/rat-cd90-antibody-ox-7-mca47.html?f=purified&JSESSIONID_STERLING=0A437413D342F782A4229D2A2AD8CE5A.ecommerce1&thirdPartyCookieEnabled=false)** > > Mouse anti Rat CD90 antibody, clone OX-7 recognizes rat and CD90, also known as Thy1.1, a GPI-anchored membrane protein containing a single V type Ig-like domain CD90 is expressed on … [https://www.stemcell.com/neurofluor-neuo.html](https://www.stemcell.com/neurofluor-neuo.html) I messed around with the NeuO dye. It’s not as selective as I wanted. Bu those are options I’m aware of. You could also possibly try NeuroTrace. I’ve never used it on dissociated cells but it may work. ---
**Author:** ![ShanTan](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/shantan/32/242_2.png) [@ShanTan](https://forum.painresearcher.net/u/ShanTan)\ **Post date:** [August 24, 2021, 8:37pm UTC](https://forum.painresearcher.net/t/facs-neurons-and-astrocytes/877/4 "2021-08-24T20:37:28Z")
> [@thicunha](#): > > ting of astrocytes in the spinal cord of mice using anti-ACSA2 antibody anti-ACSA2 - PE (IH3-18A3, Miltenyi Biotec, 1:250). Neurons are more complicated due to viability. You can find more information in our preprint paper Thanks @thicunha! Will definitely give your protocol a try. ---
**Author:** ![ShanTan](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/shantan/32/242_2.png) [@ShanTan](https://forum.painresearcher.net/u/ShanTan)\ **Post date:** [August 24, 2021, 8:38pm UTC](https://forum.painresearcher.net/t/facs-neurons-and-astrocytes/877/5 "2021-08-24T20:38:40Z")
@achamess I’d be attempting to sort neurons from spinal cord tissue. Do you think it’s better to get a transgenic line to label neurons? I guess the labelling of the neurons themselves isn’t the issue, it’s keeping them alive and getting enough of them from tissue to be able to do anything? ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [August 25, 2021, 1:15am UTC](https://forum.painresearcher.net/t/facs-neurons-and-astrocytes/877/6 "2021-08-25T01:15:56Z")
> **[Neuronal atlas of the dorsal horn defines its architecture and links sensory...](https://pubmed.ncbi.nlm.nih.gov/29686262/)** > > The dorsal horn of the spinal cord is critical to processing distinct modalities of noxious and innocuous sensation, but little is known of the neuronal subtypes involved, hampering efforts to deduce principles governing somatic sensation. Here we... This paper uses transgenic mice to select spinal neurons. They used adolescent mice. and they had to sort and select because neuron viability is an issue. Spinal neurons are hard to extract from all their milieu without beating them up. But it’s possible.