# Fos staining in the spinal cord

**URL:** <https://forum.painresearcher.net/t/fos-staining-in-the-spinal-cord/592>\
**Category:** Experimental Techniques\
**Created:** [April 15, 2019, 11:54pm UTC](https://forum.painresearcher.net/t/fos-staining-in-the-spinal-cord/592 "2019-04-15T23:54:04Z")\
**Posts on this page:** 1\
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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [April 16, 2019, 12:52am UTC](https://forum.painresearcher.net/t/fos-staining-in-the-spinal-cord/592/2 "2019-04-16T00:52:52Z")

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> [@FosGFP Mice for Noxious Stimulation - Some results](https://forum.painresearcher.net/t/fosgfp-mice-for-noxious-stimulation-some-results/213):
>
> For anyone who is interested, I recently piloted using noxious heat stimulation (55C for 30 s) in a FosGFP animal (Barth 2004). [https://www.jax.org/strain/014135](https://www.jax.org/strain/014135) Looks pretty good. Fos IHC is superior no doubt, but that has been reported. For electrophysiology, this is useful. I perfused 2 hours after stimulation.

Standard IHC staining. Nothing special. This is the Santa Cruz Fos antibody which isn’t made anymore. 90 mins to 2 hrs is typical time.

Do you have an antibody?

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