# FosGFP Mice for Noxious Stimulation - Some results **URL:** \ **Category:** Uncategorized\ **Created:** [July 3, 2017, 2:25pm UTC](https://forum.painresearcher.net/t/fosgfp-mice-for-noxious-stimulation-some-results/213 "2017-07-03T14:25:01Z")\ **Posts on this page:** 4\ **Page:** 1
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [July 3, 2017, 2:25pm UTC](https://forum.painresearcher.net/t/fosgfp-mice-for-noxious-stimulation-some-results/213/1 "2017-07-03T14:25:01Z")
For anyone who is interested, I recently piloted using noxious heat stimulation (55C for 30 s) in a FosGFP animal (Barth 2004). [https://www.jax.org/strain/014135](https://www.jax.org/strain/014135) Looks pretty good. Fos IHC is superior no doubt, but that has been reported. For electrophysiology, this is useful. I perfused 2 hours after stimulation. ![](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/3ee7ab0e529dae791cce2c5dbe7f82d32b6f030e.jpg) ---
**Author:** ![RPSeal](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/rpseal/32/577_2.png) [@RPSeal](https://forum.painresearcher.net/u/RPSeal)\ **Post date:** [July 3, 2017, 3:34pm UTC](https://forum.painresearcher.net/t/fosgfp-mice-for-noxious-stimulation-some-results/213/2 "2017-07-03T15:34:50Z")
Is that the PC-38 antibody for cFos? GFP looks nice. What did without heat stimulation look like? Thanks for sharing this. ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [July 3, 2017, 3:40pm UTC](https://forum.painresearcher.net/t/fosgfp-mice-for-noxious-stimulation-some-results/213/3 "2017-07-03T15:40:22Z")
@RPSeal Contra side and also no stimulus control had very little signal. Maybe a single random GFP+ cell. Also, I should add that this is the native GFP fluorescence. I’m sure it would look much better with anti-GFP antibody, but that would be unrealistic for using it live. This is the Santa Cruz goat anti-Fos sc-52 (the original one, before they stopped selling it). This is also 14 um mounted slice, not free floating. Overall I’m satisfied with this. ---
**Author:** ![RPSeal](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/rpseal/32/577_2.png) [@RPSeal](https://forum.painresearcher.net/u/RPSeal)\ **Post date:** [July 3, 2017, 3:43pm UTC](https://forum.painresearcher.net/t/fosgfp-mice-for-noxious-stimulation-some-results/213/4 "2017-07-03T15:43:14Z")
Ok. Sounds good! Thanks again Alex. Very nice.