# IHC for glial activation

**URL:** <https://forum.painresearcher.net/t/ihc-for-glial-activation/49>\
**Category:** Experimental Techniques\
**Created:** [July 6, 2016, 8:24pm UTC](https://forum.painresearcher.net/t/ihc-for-glial-activation/49 "2016-07-06T20:24:01Z")\
**Posts on this page:** 1\
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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [July 6, 2016, 8:30pm UTC](https://forum.painresearcher.net/t/ihc-for-glial-activation/49/2 "2016-07-06T20:30:03Z")

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@LegakisL Good question. For astrocytes, you can use GFAP. See the [Antibody Database](http://forum.painresearcher.net/t/antibodies-for-pain-research-database/30) for good GFAP antibodies. For microglia, Iba1 is typically used, as you say, and in addition to the most popular rabbit Iba1 ab from Wako, @tberta also has found a good one from Abcam made in goat (see Database). Ox42 is also used for microglia, but I haven’t used it myself. [Anti-CD68 from Ab Serotec](https://www.bio-rad-antibodies.com/mouse-cd68-antibody-fa-11-mca1957.html) is quite good. I use at a concentration of 1:1000 for macrophages in the DRG and nerve tissue. You could try in the spinal cord too, since CD68 is thought of as a marker of activated macrophages/microglia, even though resting state cells also have. @tberta knows a lot about glial activation, so hopefully he can chime in.

I haven’t used an iNOS antibody for M1/M2 transition, but hopefully someone here can suggest a good marker.

Good luck!

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