# Lentiviral expression of sodium channels?
**URL:** https://forum.painresearcher.net/t/lentiviral-expression-of-sodium-channels/1072
**Category:** Uncategorized
**Created:** [March 4, 2023, 2:05am UTC](https://forum.painresearcher.net/t/lentiviral-expression-of-sodium-channels/1072 "2023-03-04T02:05:40Z")
**Posts on this page:** 2
**Page:** 1
### Author:  [@achamess](https://forum.painresearcher.net/u/achamess)
#### Post date: [March 4, 2023, 2:05am UTC](https://forum.painresearcher.net/t/lentiviral-expression-of-sodium-channels/1072/1 "2023-03-04T02:05:40Z")
Has anyone seen delivery of sodium channels (SCN9A, SCN10A, etc.) to primary cells (e.g. DRG culture) or cell lines via lentivirus? I haven’t come up with anything. I think the SCN genes, which are ~6K bases, might be too large. @AubinMoutal @mamocol @tberta @thicunha
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### Author:  [@AubinMoutal](https://forum.painresearcher.net/u/AubinMoutal)
#### Post date: [March 4, 2023, 3:16am UTC](https://forum.painresearcher.net/t/lentiviral-expression-of-sodium-channels/1072/2 "2023-03-04T03:16:31Z")
yes! we did this to deliver mutated channels and then record the effect of mutations on currents. all in native DRG neurons. ([Identification and targeting of a unique NaV1.7 domain driving chronic pain | bioRxiv](https://www.biorxiv.org/content/10.1101/2022.07.09.499431v1))
It was via electroporation, no viruses. I have the machine in my lab if you want to try it! with electroporation, we reliably get 20-30% of DRGs transfected.
I didn’t try lentivirus but this could be within packaging capacity with a good desing of your vector. My vector for CRISPR is giant and could be put into lentiviruses no problem!