# Lentivirus for in vitro adult DRG transduction

**URL:** <https://forum.painresearcher.net/t/lentivirus-for-in-vitro-adult-drg-transduction/913>\
**Category:** Experimental Techniques\
**Created:** [December 16, 2021, 6:46pm UTC](https://forum.painresearcher.net/t/lentivirus-for-in-vitro-adult-drg-transduction/913 "2021-12-16T18:46:38Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [December 16, 2021, 6:46pm UTC](https://forum.painresearcher.net/t/lentivirus-for-in-vitro-adult-drg-transduction/913/1 "2021-12-16T18:46:38Z")

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Has anyone had success using lentivirus on adult primary DRG cultures for transgene infection?

@tberta @liz @dmolliver

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**Author:** ![dmolliver](https://avatars.discourse-cdn.com/v4/letter/d/5e9695/32.png) [@dmolliver](https://forum.painresearcher.net/u/dmolliver)\
**Post date:** [January 10, 2022, 4:44pm UTC](https://forum.painresearcher.net/t/lentivirus-for-in-vitro-adult-drg-transduction/913/2 "2022-01-10T16:44:53Z")

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I would be shocked if someone hasn’t done this, although I can’t think of a paper offhand. I did just happen to come across this study using lenti in embryonic neurons (PMID: 27486387), I realize that isn’t that helpful for adult. Of course, AAV and HSV both seem to work well for adult DRG.