# Mouse age for neuropathic pain induction **URL:** \ **Category:** Animal Behavior and Models\ **Created:** [July 27, 2022, 1:54am UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003 "2022-07-27T01:54:17Z")\ **Posts on this page:** 10\ **Page:** 1
**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\ **Post date:** [July 27, 2022, 1:54am UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/1 "2022-07-27T01:54:17Z")
What is the best age of mouse to induce neuropathic pain, specially SNI? Anyone has compared the age of mice? Any difference between 7-10 weeks old compared with older (15-20 weeks?) @achamess @tberta @jmogil @Theodore.price ---
**Author:** ![jmogil](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/jmogil/32/7_2.png) [@jmogil](https://forum.painresearcher.net/u/jmogil)\ **Post date:** [July 27, 2022, 1:56pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/2 "2022-07-27T13:56:11Z")
There’s very little difference between a 7-10 week old and a 15-20 week old mouse. I’d be hugely surprised if anyone ever saw any difference, especially using the extremely robust SNI. We compared 8-week-old to 12-MONTH old mice, and found no difference in SNI mechanical allodynia two weeks after surgery (Muralidharan et al., Pain Rep. 5:e824, 2020). ---
**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\ **Post date:** [July 27, 2022, 2:41pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/3 "2022-07-27T14:41:56Z")
Hi @jmogil. Thanks. In terms of pain hypersensitivity, I agree that it is difficult to see differences since SNI is extremely robust. What we are seeing is a huge difference in the immune response when comparing young mice (7-12 weeks) with adult mice (~ 20 weeks or older). ---
**Author:** ![jmogil](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/jmogil/32/7_2.png) [@jmogil](https://forum.painresearcher.net/u/jmogil)\ **Post date:** [July 27, 2022, 2:53pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/4 "2022-07-27T14:53:42Z")
How very interesting! ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [July 29, 2022, 2:22pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/5 "2022-07-29T14:22:38Z")
Very interesting. Yeah I would not have thought there were would be much biological difference between mice that close in age. Keep us posted @thicunha ---
**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\ **Post date:** [July 29, 2022, 2:49pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/6 "2022-07-29T14:49:23Z")
We don’t know yet if these differences are intrinsically related to age or if SPF animals start to maturate better their immune system in a conventional facility. We usually receive SPF animals at 6 weeks old and start SNI with 7-9 weeks old. So, our older mice (over 14 weeks) are in a conventional experimental facility. So, there is a bias on this point and it isn’t easy to compare. ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [August 6, 2022, 5:50pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/7 "2022-08-06T17:50:07Z")
> **[Sex-specific effects of neuropathic pain on long-term pain... : PAIN](https://journals.lww.com/pain/Abstract/9900/Sex_specific_effects_of_neuropathic_pain_on.138.aspx)** > > opathic pain. Mice were weighed monthly, tested behaviorally for mechanical and cold sensitivity and guarding behavior every three months post-surgery, and otherwise left undisturbed in their cages until death by natural causes. Evidence of pain over... A related question. How long does the effect of SNI last? @jmogil nice study! ---
**Author:** ![jmogil](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/jmogil/32/7_2.png) [@jmogil](https://forum.painresearcher.net/u/jmogil)\ **Post date:** [August 8, 2022, 2:49pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/8 "2022-08-08T14:49:14Z")
I’m not sure what you mean by “effect”. The allodynia over the lifespan is in Figure 2. ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [August 8, 2022, 3:29pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/9 "2022-08-08T15:29:59Z")
Sorry. I wrote too quickly. Didn’t mean to phrase as a question. I meant to say your study reports on the duration of SNI’s behavioral effects, as opposed to any differences in age of onset, which was Thiago questions. Anyway, important study and interesting findings. Thanks for doing it! ---
**Author:** ![jmogil](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/jmogil/32/7_2.png) [@jmogil](https://forum.painresearcher.net/u/jmogil)\ **Post date:** [August 8, 2022, 3:41pm UTC](https://forum.painresearcher.net/t/mouse-age-for-neuropathic-pain-induction/1003/10 "2022-08-08T15:41:36Z")
Gotcha. Thanks for saying!