# Pax2 antibody help **URL:** \ **Category:** Microscopy (IHC/ISH/ICC/In Vivo)\ **Created:** [January 18, 2018, 5:33am UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383 "2018-01-18T05:33:55Z")\ **Posts on this page:** 7\ **Page:** 1
**Author:** ![ShanTan](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/shantan/32/242_2.png) [@ShanTan](https://forum.painresearcher.net/u/ShanTan)\ **Post date:** [January 18, 2018, 5:33am UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383/1 "2018-01-18T05:33:55Z")
Wondering if anyone has had successful staining with Pax2 antibodies? I’ve tried a rabbit polyclonal antibody from abcam, and a goat polyclonal from R&D systems. I’ve done concentration curves, but I’m finding that even at the lowest concentrations (1:2000), most neurons still seem to be staining for Pax2 (costaining with NeuN). I can reduce concentration even more…just wondering if maybe the antibodies I’m using are no good. Some neurons appear less bright than others, but from what I had read in the literature, it should be clearly positive with Pax2, or nothing at all. Any help on this would be greatly appreciated! ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [January 18, 2018, 1:24pm UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383/2 "2018-01-18T13:24:31Z")
@ShanTan > **[PAX2 Polyclonal Antibody (71-6000)](https://www.thermofisher.com/antibody/product/PAX2-Antibody-Polyclonal/71-6000)** > > Invitrogen Anti-PAX2 Polyclonal, Catalog # 71-6000. Tested in Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP) and ELISA (ELISA) applications. This antibody reacts with Human, Mouse, Ovine, Rat, Xenopus laevis, Zebrafish... 1:500. This is the good one. Sorry you tried those other ones first. You can see it here in one of my image panels: > [@Stuff I tried during grad school/post-doc that won't get published but could be useful](http://forum.painresearcher.net/t/stuff-i-tried-during-grad-school-post-doc-that-wont-get-published-but-could-be-useful/302/6): > > I talked about this in another thread Here are some more images. Overall, this FosGFP line is good for activity-dependent labeling. It’s not perfect. There are some Fos+ (IHC) neurons that don’t get labeled, but overall, it’s useful. Could be good for electrophysiology. The stimulus here was 55ºC heat for 30s, followed by perfusion 2 hours after the stimulus. [fos-slices-panel-01-01] Also, don’t fix too long. Light fixation. 1-2h post fix after your PFA perfusion. ---
**Author:** ![zhzhj131421](https://avatars.discourse-cdn.com/v4/letter/z/f6c823/32.png) [@zhzhj131421](https://forum.painresearcher.net/u/zhzhj131421)\ **Post date:** [January 23, 2018, 11:00am UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383/3 "2018-01-23T11:00:46Z")
I think the rabbit anti-PAX2 antibody from Thermo Fisher Scientific Cat#716000 is better. ![image](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/99b424992a0491428ed5ac50cc354aac87d5a40c.jpg) is spinal dorsal horn ---
**Author:** ![zhzhj131421](https://avatars.discourse-cdn.com/v4/letter/z/f6c823/32.png) [@zhzhj131421](https://forum.painresearcher.net/u/zhzhj131421)\ **Post date:** [January 23, 2018, 11:02am UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383/4 "2018-01-23T11:02:46Z")
I bought this antibody, this picture is from our lab. ---
**Author:** ![zhzhj131421](https://avatars.discourse-cdn.com/v4/letter/z/f6c823/32.png) [@zhzhj131421](https://forum.painresearcher.net/u/zhzhj131421)\ **Post date:** [January 23, 2018, 11:04am UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383/5 "2018-01-23T11:04:09Z")
Who knows which company has a good TLX3 antibody? ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [January 23, 2018, 11:23am UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383/6 "2018-01-23T11:23:31Z")
@zhzhj131421 The TLX3 ab thay everyone uses in papers is from Carmen Birchmaer’s Lab in Germany. Ask if they will send you some. I asked and they were very generous. But it’s be nice if there were a good commercial one. ---
**Author:** ![zhzhj131421](https://avatars.discourse-cdn.com/v4/letter/z/f6c823/32.png) [@zhzhj131421](https://forum.painresearcher.net/u/zhzhj131421)\ **Post date:** [January 23, 2018, 11:39am UTC](https://forum.painresearcher.net/t/pax2-antibody-help/383/7 "2018-01-23T11:39:34Z")
Thanks, ALEX. I will try the TLX3 antibody from GeneTex Company.