# Pgp9.5 glabrous skin staining

**URL:** <https://forum.painresearcher.net/t/pgp9-5-glabrous-skin-staining/177>\
**Category:** Protocols\
**Created:** [February 11, 2017, 1:46pm UTC](https://forum.painresearcher.net/t/pgp9-5-glabrous-skin-staining/177 "2017-02-11T13:46:08Z")\
**Posts on this page:** 1\
**Page:** 2

<div class="post-metadata">

**Author:** ![Chunny](https://avatars.discourse-cdn.com/v4/letter/c/f1d935/32.png) [@Chunny](https://forum.painresearcher.net/u/Chunny)\
**Post date:** [July 10, 2024, 5:02am UTC](https://forum.painresearcher.net/t/pgp9-5-glabrous-skin-staining/177/21 "2024-07-10T05:02:33Z")

</div>

@achamess Hi, is your protocol to cut only the circled part of the mouse paw (as shown in the attached picture)? Thanks for your reply.  
 ![20240627221401](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/4c97a44320993a30124181bfcf14f73d2d476a2b.png)

@MGeron Hi! I would like to tell you the good news that I have achieved excellent results in skin tissues fixed to slides using proteintech’s PGP9.5, both methanol acetone method fixation and zamboni fixation. I also tried the floating slice method and I wonder if I don’t have a good tool and always lose the slides. Good luck!

[Previous page](https://forum.painresearcher.net/t/pgp9-5-glabrous-skin-staining/177.md?page=1)