# Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine

**URL:** <https://forum.painresearcher.net/t/preparation-of-acute-brain-slices-using-an-optimized-n-methyl-d-glucamine/415>\
**Category:** Uncategorized\
**Created:** [March 13, 2018, 9:11pm UTC](https://forum.painresearcher.net/t/preparation-of-acute-brain-slices-using-an-optimized-n-methyl-d-glucamine/415 "2018-03-13T21:11:23Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [March 13, 2018, 9:11pm UTC](https://forum.painresearcher.net/t/preparation-of-acute-brain-slices-using-an-optimized-n-methyl-d-glucamine/415/1 "2018-03-13T21:11:23Z")

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This excellent video from the Allen Institute has a lot of useful information and procedures. Many people like the NMDG method for brain slices, but the original protocol was not optimal for young animals, as are frequently used in spinal cord slice studies. However, the new protocol here with Na+ spike-in circumvents this limitation, making this protocol suitable for young animals as well.

Also of note, this demonstrates how to use the @compresstome slicer, which we’ve discussed here before.

> [@Improve spinal cord and brain slice preparation with the Compresstome® vibratome (Sponsored Content)](https://forum.painresearcher.net/t/improve-spinal-cord-and-brain-slice-preparation-with-the-compresstome-vibratome-sponsored-content/287):
>
> Improve spinal cord and brain slice preparation with the Compresstome® vibratome For many of you who are devoted to pain research, it’s likely that you need to make tissue slices of the brain or spinal cord. What do you use for making healthy lives slices for [electrophysiology](https://precisionary.com/electrophysiology/) ? What do you use for creating smooth, thin sections for [immuno](https://precisionary.com/immunohistochemistry/)? [image-1-cutter] Compresstome® tissue slicers are affordable, compact, and produce healthier live spinal cord slices compared to other market vibratomes. E…

[https://www.jove.com/video/53825/preparation-acute-brain-slices-using-an-optimized-n-methyl-d](https://www.jove.com/video/53825/preparation-acute-brain-slices-using-an-optimized-n-methyl-d)

@jordangmccall @cedric.peirs @RPSeal @Priyabrata @liz