# Primary mouse DRG immunocytochemistry troubleshooting - cell detachment **URL:** \ **Category:** Uncategorized\ **Created:** [August 6, 2023, 7:42pm UTC](https://forum.painresearcher.net/t/primary-mouse-drg-immunocytochemistry-troubleshooting-cell-detachment/1126 "2023-08-06T19:42:54Z")\ **Posts on this page:** 1\ **Showing post:** 5
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [December 2, 2023, 1:52pm UTC](https://forum.painresearcher.net/t/primary-mouse-drg-immunocytochemistry-troubleshooting-cell-detachment/1126/5 "2023-12-02T13:52:27Z")
HI @dmolliver Thanks for checking in on this. We figured it out, but can’t be entirely sure what. but I went back to basics. I got new, fresh laminin from Sigma. and as you say above, instead of letting the laminin dry, we went right into wells immediately after aspirating, following this protocol [Protocol for dissection and culture of murine dorsal root ganglia neurons to study neuropeptide release - PubMed](https://pubmed.ncbi.nlm.nih.gov/33615276/) It seems to have worked. We had abundant neurons on the glass this time. ![image](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/edbe3e2c04899de45be2d8b4da4536cc01ffdb8b.jpeg) So my take away: 1. Fresh reagents including PDL and laminin 2. Don’t let laminin dry --- _[View the full topic](https://forum.painresearcher.net/t/primary-mouse-drg-immunocytochemistry-troubleshooting-cell-detachment/1126)._