# Pulling glass pipettes for spinal cord viral injections

**URL:** <https://forum.painresearcher.net/t/pulling-glass-pipettes-for-spinal-cord-viral-injections/210>\
**Category:** Experimental Techniques\
**Created:** [June 5, 2017, 4:10pm UTC](https://forum.painresearcher.net/t/pulling-glass-pipettes-for-spinal-cord-viral-injections/210 "2017-06-05T16:10:46Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [June 5, 2017, 4:10pm UTC](https://forum.painresearcher.net/t/pulling-glass-pipettes-for-spinal-cord-viral-injections/210/1 "2017-06-05T16:10:46Z")

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Hi all,  
Can anyone here recommend some parameters for making glass capillaries for injecting into the spinal cord? If you use a puller (e.g. Sutter P1000 or P-97), can you recommend settings? Or what diameter bore size are you looking for? And any preferences on capillaries (vendor, size, etc.)?

@RPSeal @zhzhj131421 @luovirus @tberta @liz

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [June 9, 2017, 3:21pm UTC](https://forum.painresearcher.net/t/pulling-glass-pipettes-for-spinal-cord-viral-injections/210/2 "2017-06-09T15:21:11Z")

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FYI: Here is a recipe from @zhzhj131421 : [http://www.bio-protocol.org/e2020](http://www.bio-protocol.org/e2020)

> Prepare the glass capillary: the glass capillaries are pulled by a P-97 micropipette puller with the heat: 850, vel: 30, and time: 250.  
> Trim the tip of the glass capillary tube with scissors under operating microscope, so that the tapered tip has an outer diameter of about 50 μm.  
> Insert the tip of glass microliter syringe (type of volume 10μl) into the glass capillary, and seal the junction with hot glue (Video 1).  
> Fill the glass microliter syringe and glass capillary with liquid paraffin, evacuate the air in them.  
> Fix the microinjection set on the desktop digital stereotaxic instruments.