# Recommendation for ATF3 antibody (not Santa Cruz) **URL:** \ **Category:** Reagents and Equipment\ **Created:** [May 7, 2019, 9:13pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599 "2019-05-07T21:13:35Z")\ **Posts on this page:** 20\ **Page:** 1
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [May 7, 2019, 9:13pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/1 "2019-05-07T21:13:35Z")
Does anyone have recommendations for an ATF3 antibody? The Santa Cruz one was excellent but is no longer available. @tberta @liz ---
**Author:** ![CandlerPaige](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/candlerpaige/32/250_2.png) [@CandlerPaige](https://forum.painresearcher.net/u/CandlerPaige)\ **Post date:** [May 7, 2019, 9:27pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/2 "2019-05-07T21:27:51Z")
@sshiers have any suggestions? ---
**Author:** ![sshiers](https://avatars.discourse-cdn.com/v4/letter/s/e9c0ed/32.png) [@sshiers](https://forum.painresearcher.net/u/sshiers)\ **Post date:** [May 7, 2019, 9:43pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/3 "2019-05-07T21:43:51Z")
Is the one from Santa Cruz a mouse monoclonal? ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [May 7, 2019, 9:47pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/4 "2019-05-07T21:47:02Z")
This was the one that worked well: [https://www.scbt.com/scbt/product/atf-3-antibody-c-19](https://www.scbt.com/scbt/product/atf-3-antibody-c-19) No longer available. ---
**Author:** ![tberta](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/tberta/32/145_2.png) [@tberta](https://forum.painresearcher.net/u/tberta)\ **Post date:** [May 7, 2019, 9:59pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/5 "2019-05-07T21:59:57Z")
We are considering to try the following one: [https://www.novusbio.com/products/atf3-antibody\_nbp1-85816](https://www.novusbio.com/products/atf3-antibody_nbp1-85816) We will keep you posted! ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [May 8, 2019, 3:07pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/6 "2019-05-08T15:07:53Z")
Thanks @tberta. Please keep us posted. ---
**Author:** ![ShanTan](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/shantan/32/242_2.png) [@ShanTan](https://forum.painresearcher.net/u/ShanTan)\ **Post date:** [May 15, 2019, 3:24pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/7 "2019-05-15T15:24:45Z")
@achamess There is a rabbit monoclonal from abcam that works great. [https://www.abcam.com/atf3-antibody-epr19488-ab207434.html](https://www.abcam.com/atf3-antibody-epr19488-ab207434.html) ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [May 15, 2019, 3:26pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/8 "2019-05-15T15:26:10Z")
Awesome! Thanks @ShanTan ---
**Author:** ![gcorder](https://avatars.discourse-cdn.com/v4/letter/g/c67d28/32.png) [@gcorder](https://forum.painresearcher.net/u/gcorder)\ **Post date:** [May 20, 2019, 5:54pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/9 "2019-05-20T17:54:51Z")
We’re about to test out the Abcam rabbit on DRG and SC this week … also, has anyone looked at ATF3 in the brain after a peripheral injury? I saw in the ATF3-CreERT2 mouse paper ([http://www.eneuro.org/content/6/2/ENEURO.0025-19.2019](http://www.eneuro.org/content/6/2/ENEURO.0025-19.2019)) that they showed a qualitative image of ATF3 in PVT, but I couldn’t find other examples … ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [June 24, 2020, 3:33pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/10 "2020-06-24T15:33:04Z")
@sshiers Have you found a good ATF3 antibody? ---
**Author:** ![sshiers](https://avatars.discourse-cdn.com/v4/letter/s/e9c0ed/32.png) [@sshiers](https://forum.painresearcher.net/u/sshiers)\ **Post date:** [August 28, 2020, 2:38pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/11 "2020-08-28T14:38:28Z")
Never tried ATF3 antibodies before ---
**Author:** ![mpertin](https://avatars.discourse-cdn.com/v4/letter/m/9e8a1a/32.png) [@mpertin](https://forum.painresearcher.net/u/mpertin)\ **Post date:** [March 3, 2021, 1:50pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/12 "2021-03-03T13:50:28Z")
We changed the santa cruz ATF3 for the Abcam one. ref ab207434. it works well! ---
**Author:** ![mpertin](https://avatars.discourse-cdn.com/v4/letter/m/9e8a1a/32.png) [@mpertin](https://forum.painresearcher.net/u/mpertin)\ **Post date:** [March 3, 2021, 1:55pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/13 "2021-03-03T13:55:08Z")
I forgot to say that we also tested the abcam ab180842 and it was not working in our hands. ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [March 3, 2021, 8:10pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/14 "2021-03-03T20:10:06Z")
Thank you @mpertin for the news. This is very helpful. Would you be willing to share some images? I’m glad you were able to find a suitable replacement for the Santa Cruz antibody. Also, welcome to the Pain Researcher forum! ---
**Author:** ![mpertin](https://avatars.discourse-cdn.com/v4/letter/m/9e8a1a/32.png) [@mpertin](https://forum.painresearcher.net/u/mpertin)\ **Post date:** [March 4, 2021, 9:27am UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/15 "2021-03-04T09:27:57Z")
Sure! here it is. DRG L5 form SNL mice ![image](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/740ac122c72666b36c5e00b93a389b9ccf71b6e2.png) ---
**Author:** ![mpertin](https://avatars.discourse-cdn.com/v4/letter/m/9e8a1a/32.png) [@mpertin](https://forum.painresearcher.net/u/mpertin)\ **Post date:** [March 4, 2021, 9:33am UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/16 "2021-03-04T09:33:26Z")
and a "SNL"Sham L5 ![image](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/f37ba65cf3ba5967ec9cc9e6cad4b9863de04e59.png) ---
**Author:** ![mpertin](https://avatars.discourse-cdn.com/v4/letter/m/9e8a1a/32.png) [@mpertin](https://forum.painresearcher.net/u/mpertin)\ **Post date:** [March 4, 2021, 9:36am UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/17 "2021-03-04T09:36:49Z")
and we had no signal at all in the Naive DRG. Was also great in blue wit AF350 antibody. We also test the Thermofisher one Catalog Number PA5-36244 and the Abcam ab180842 both of them were NOT working ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [March 4, 2021, 1:21pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/18 "2021-03-04T13:21:59Z")
@mpertin: Thank you! This is wonderful. You will save many labs time and money because you so generously shared your experience here. This is how science should be. The images look great! Interesting how much injury still occurs in the sham. Well done. ---
**Author:** ![mpertin](https://avatars.discourse-cdn.com/v4/letter/m/9e8a1a/32.png) [@mpertin](https://forum.painresearcher.net/u/mpertin)\ **Post date:** [March 4, 2021, 1:38pm UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/19 "2021-03-04T13:38:48Z")
with pleasure!!! Yes the sham for SNL model in our hands gives quite some ATF3 signal also…even if it is a sham it is quite an invasive procedure still (open muscle and break bones). but it also depend from the skills of the person doing the surgery. ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [September 18, 2021, 2:16am UTC](https://forum.painresearcher.net/t/recommendation-for-atf3-antibody-not-santa-cruz/599/20 "2021-09-18T02:16:41Z")
Hi all. I used the ATF3 antibody that @mpertin recommended at 1:500 and it looked great. Also I tried a c-Jun antibody on DRG from sciatic nevre crush at POD1. Looks great! 1:250. ![image](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/f630fd39dec10dabfb13544e1aa34b830fa6fd08.jpeg) > **[c-Jun (60A8) Rabbit mAb](https://www.cellsignal.com/products/primary-antibodies/c-jun-60a8-rabbit-mab/9165)** > > Monoclonal Antibody for studying JUN in the research area.