# RNAscope (In situ hybridization) to study gene expression in pain-related tissues

**URL:** <https://forum.painresearcher.net/t/rnascope-in-situ-hybridization-to-study-gene-expression-in-pain-related-tissues/102>\
**Category:** Experimental Techniques\
**Created:** [September 13, 2016, 11:59am UTC](https://forum.painresearcher.net/t/rnascope-in-situ-hybridization-to-study-gene-expression-in-pain-related-tissues/102 "2016-09-13T11:59:43Z")\
**Posts on this page:** 1\
**Showing post:** 24

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**Author:** ![MGM](https://avatars.discourse-cdn.com/v4/letter/m/ebca7d/32.png) [@MGM](https://forum.painresearcher.net/u/MGM)\
**Post date:** [August 16, 2018, 5:30pm UTC](https://forum.painresearcher.net/t/rnascope-in-situ-hybridization-to-study-gene-expression-in-pain-related-tissues/102/24 "2018-08-16T17:30:01Z")

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I am a colleague of achamess and adding some information about sample pretreatment.

> [@june](#):
>
> For sample pretreatment, do you skip the hydrogen peroxide step prior to protease IV when using the v2 kit?

We use both hydrogen peroxide (10min@RT) and proteaseIV (30min@RT) as pretreatment for the v2 kit.

Good luck:grinning:

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