# Simplified Production of Adeno-Associated Virus from HEK293T Cells

**URL:** <https://forum.painresearcher.net/t/simplified-production-of-adeno-associated-virus-from-hek293t-cells/28>\
**Category:** Protocols\
**Created:** [June 27, 2016, 6:12pm UTC](https://forum.painresearcher.net/t/simplified-production-of-adeno-associated-virus-from-hek293t-cells/28 "2016-06-27T18:12:10Z")\
**Posts on this page:** 1\
**Page:** 1

<div class="post-metadata">

**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [June 27, 2016, 6:12pm UTC](https://forum.painresearcher.net/t/simplified-production-of-adeno-associated-virus-from-hek293t-cells/28/1 "2016-06-27T18:12:10Z")

</div>

Here is a simplified protocol that I use to make adeno-associated virus (AAV) in house. It’s based on protocols from [Lock et al. 2010](http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2957274/) and [Vandenberghe et al 2010](http://www.ncbi.nlm.nih.gov/pubmed/20649475). We generally make AAV9 serotype, which is released very efficiently into the medium, so that is why we collect the medium here.

If you have any questions, let me know.

[AAV Protocol](https://dl.dropboxusercontent.com/u/6341114/PainResearcher_Resources/aav-production-vdv-lab-protocol-v1.pdf)