# Simultaneous Cryosectioning of Multiple Rodent Brains | Protocol

**URL:** https://forum.painresearcher.net/t/simultaneous-cryosectioning-of-multiple-rodent-brains-protocol/499
**Category:** Protocols
**Created:** [November 1, 2018, 8:48pm UTC](https://forum.painresearcher.net/t/simultaneous-cryosectioning-of-multiple-rodent-brains-protocol/499 "2018-11-01T20:48:34Z")
**Posts on this page:** 1
**Page:** 1

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### Author: ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)
#### Post date: [November 1, 2018, 8:48pm UTC](https://forum.painresearcher.net/t/simultaneous-cryosectioning-of-multiple-rodent-brains-protocol/499/1 "2018-11-01T20:48:34Z")

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> **[Simultaneous Cryosectioning of Multiple Rodent Brains | Protocol](https://www.jove.com/video/58513/simultaneous-cryosectioning-of-multiple-rodent-brains)**
>
> Here, we present a protocol to freeze and section brain tissue from multiple animals as a timesaving alternative to processing single...

I thought this was pretty cool. And it also shows a way to solve a problem I mentioned previously - the bubbles on the slide. They use a PBS-wetted paint brush to brush out the bubbles. And they keep the slide warm on a slide warmer so that the OCT doesn’t dry immediately.