# SOX9 as an Astrocyte Marker: Has anyone used in IHC or FACS?

**URL:** <https://forum.painresearcher.net/t/sox9-as-an-astrocyte-marker-has-anyone-used-in-ihc-or-facs/374>\
**Category:** Experimental Techniques\
**Tags:** glia, ihc\
**Created:** [January 8, 2018, 10:02pm UTC](https://forum.painresearcher.net/t/sox9-as-an-astrocyte-marker-has-anyone-used-in-ihc-or-facs/374 "2018-01-08T22:02:48Z")\
**Posts on this page:** 1\
**Page:** 1

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [January 8, 2018, 10:02pm UTC](https://forum.painresearcher.net/t/sox9-as-an-astrocyte-marker-has-anyone-used-in-ihc-or-facs/374/1 "2018-01-08T22:02:48Z")

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I saw this thorough paper (Sun et al. 2017) where they identified Sox9 as an astrocyte-specific marker.

> **[SOX9 Is an Astrocyte-Specific Nuclear Marker in the Adult Brain Outside the...](https://www.jneurosci.org/content/37/17/4493)**
>
> Astrocytes have in recent years become the focus of intense experimental interest, yet markers for their definitive identification remain both scarce and imperfect. Astrocytes may be recognized as such by their expression of glial fibrillary acidic...

![sun2017_fig2](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/23439dd85a952912d2c0fa3e212c35101790aaa0.jpg)

Has anyone used this? They use AF3075 from R&D in the paper.

Looks pretty good!

@tberta
