# Stuff I tried during grad school/post-doc that won't get published but could be useful **URL:** \ **Category:** Resources\ **Created:** [October 15, 2017, 5:55pm UTC](https://forum.painresearcher.net/t/stuff-i-tried-during-grad-school-post-doc-that-wont-get-published-but-could-be-useful/302 "2017-10-15T17:55:28Z")\ **Posts on this page:** 1\ **Showing post:** 10
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [October 15, 2017, 8:26pm UTC](https://forum.painresearcher.net/t/stuff-i-tried-during-grad-school-post-doc-that-wont-get-published-but-could-be-useful/302/10 "2017-10-15T20:26:22Z")
Intrathecal AAV9 works excellently to get DRG neurons in the lumbar region. I injected the same AAV-hSyn-EGFP (10^13), 10 ul, and got very high expression. In light of the recent work looking at promoters in DRGs, I bet CAG would have been even better. However, it also hits spinal neurons and probably higher CNS areas (as reported) ![drg_dapi_10x_1](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/c3394f40e3cf7c9e868c5016665af7ac0310512e.jpg) ![lsc-20x-edf_1c1 copy](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/3d4305be07c944383ae40b58d8525bcb815ff08b.jpg) _Spinal cord_ --- _[View the full topic](https://forum.painresearcher.net/t/stuff-i-tried-during-grad-school-post-doc-that-wont-get-published-but-could-be-useful/302)._