# Stuff I tried during grad school/post-doc that won't get published but could be useful **URL:** \ **Category:** Resources\ **Created:** [October 15, 2017, 5:55pm UTC](https://forum.painresearcher.net/t/stuff-i-tried-during-grad-school-post-doc-that-wont-get-published-but-could-be-useful/302 "2017-10-15T17:55:28Z")\ **Posts on this page:** 1\ **Showing post:** 14
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [October 27, 2017, 7:04pm UTC](https://forum.painresearcher.net/t/stuff-i-tried-during-grad-school-post-doc-that-wont-get-published-but-could-be-useful/302/14 "2017-10-27T19:04:11Z")
For pan-neuronal labeling in the DRG, I’ve moved to Neurotrace, which is a fluorescent Nissl stain. The different colors have variable staining qualities. Here is Neurotrace Blue. I used 1:200 diluted in PBS for 10 mins at room temp. I do a quick wash in PBS and then mount. I’ve found the Far red Neurotrace to be good too. ![nissl-blue-1to200-drg-testC1](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/2141957c2a3ef0a8d688638e0b22037a2fd39c7b.jpg) The Green Neutrotrace is very strong, but it also stains non-neuronal nuclei too (like real Nissl). I’ve found the blue and Far red to do that less. This is 1:100 in spinal cord Nissl Green 20 mins at RT. 1x wash with PBS ![green_sc_1to100_10xc2 copy](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/5efe3a81f0d958daf24c093568269f38d9b0d6b5.jpg) Blue is nice because you don’t have to take up the green channel. That’s good since we very rarely use a secondary fluor that is blue. It’s generally FITC/488, Cy3 and Cy5 in our lab. So you can do 4 colors this way, assuming you exclude DAPI. --- _[View the full topic](https://forum.painresearcher.net/t/stuff-i-tried-during-grad-school-post-doc-that-wont-get-published-but-could-be-useful/302)._