# Testing Dynamic Allodynia with Brush or Cotton - Tips and Tricks?

**URL:** <https://forum.painresearcher.net/t/testing-dynamic-allodynia-with-brush-or-cotton-tips-and-tricks/432>\
**Category:** Animal Behavior and Models\
**Created:** [April 27, 2018, 7:02pm UTC](https://forum.painresearcher.net/t/testing-dynamic-allodynia-with-brush-or-cotton-tips-and-tricks/432 "2018-04-27T19:02:56Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [April 27, 2018, 7:02pm UTC](https://forum.painresearcher.net/t/testing-dynamic-allodynia-with-brush-or-cotton-tips-and-tricks/432/1 "2018-04-27T19:02:56Z")

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More papers are distinguish dynamic from static (von Frey) mechanical stimuli. The common assay is using a paint brush or cotton swab.

In my hands, naive mice definitely respond to either of these with a brisk lift.  
There is some variability in the application of the stimulus because the mesh that the mice stand on can sometimes get in the way of the brush/cotton.

What is everyone’s experience with this? And do you see strong changes after nerve injury the way these papers do?

A video demonstration of the proper technique AND the scoring would be a great resource too.

> **[Identification of spinal circuits transmitting and gating mechanical pain](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4258511/)**
>
> Pain processing in the spinal cord has been postulated to rely on nociceptive transmission (T) neurons receiving inputs from nociceptors and Aβ mechanoreceptors, with Aβ inputs gated through feed-forward activation of spinal inhibitory ...

  

> **[Identification of spinal circuits involved in touch-evoked dynamic mechanical...](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5470641/)**
>
> Mechanical hypersensitivity is a debilitating symptom associated with millions of chronic pain patients. It exists in distinct forms, including brush-evoked dynamic and filament-evoked punctate. Here we report that dynamic mechanical hypersensitivity...

@tberta @thicunha @esypek @liz @tonellor @zhzhj131421 @SamineniV @ShanTan @fmoehring

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**Author:** ![zhzhj131421](https://avatars.discourse-cdn.com/v4/letter/z/f6c823/32.png) [@zhzhj131421](https://forum.painresearcher.net/u/zhzhj131421)\
**Post date:** [April 29, 2018, 2:59am UTC](https://forum.painresearcher.net/t/testing-dynamic-allodynia-with-brush-or-cotton-tips-and-tricks/432/2 "2018-04-29T02:59:30Z")

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I will try to do this behavior. I will use a very soft brush to do this.