# Time course of light touch-induced pERK in Neuropathic Models

**URL:** <https://forum.painresearcher.net/t/time-course-of-light-touch-induced-perk-in-neuropathic-models/139>\
**Category:** Uncategorized\
**Created:** [December 8, 2016, 7:52pm UTC](https://forum.painresearcher.net/t/time-course-of-light-touch-induced-perk-in-neuropathic-models/139 "2016-12-08T19:52:42Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [December 8, 2016, 7:52pm UTC](https://forum.painresearcher.net/t/time-course-of-light-touch-induced-perk-in-neuropathic-models/139/1 "2016-12-08T19:52:42Z")

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I’m looking for some references for phospho-ERK induction in the dorsal horn in response to light touch stimuli (e.g. cotton) in neuropathic models, namely CCI or SNI.

For example, in the CFA model, Gao et al. used cotton brush on the hindpaw for 5 minutes and quantified pERK in the dorsal horn following stimulation.

> **[Light touch induces ERK activation in superficial dorsal horn neurons after...](https://onlinelibrary.wiley.com/doi/full/10.1111/j.1471-4159.2010.06946.x)**
>
> J. Neurochem. (2010) 115, 505–514.
>  
> Abstract
> Activation of extracellular signal‐regulated kinase (ERK) in spinal cord neurons could serve as a marker for sensitization of dorsal horn neurons in pe...

Are there comparable papers for pERK in neuropathic models? Mostly, I want to know the best time point.

@tberta @thicunha @rurongji

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**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\
**Post date:** [December 9, 2016, 1:46pm UTC](https://forum.painresearcher.net/t/time-course-of-light-touch-induced-perk-in-neuropathic-models/139/2 "2016-12-09T13:46:32Z")

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@achamess we have in our hand a significant increase in the dorsal horn 7 days after SNI

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**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\
**Post date:** [December 9, 2016, 1:47pm UTC](https://forum.painresearcher.net/t/time-course-of-light-touch-induced-perk-in-neuropathic-models/139/3 "2016-12-09T13:47:45Z")

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@achamess Sorry but it is baseline not after touch

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [December 14, 2016, 10:56pm UTC](https://forum.painresearcher.net/t/time-course-of-light-touch-induced-perk-in-neuropathic-models/139/4 "2016-12-14T22:56:17Z")

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@thicunha Thanks! Have you published that data? Or if not, could you tell me some details about your protocol?

- Mice or rats?
- What time point? (7 days)?
- What segment? (L3, L4 or L5?)
- Section and staining (free floating, 30-40 um?)

I want to make pick the time point at which the basal level is not very high so I can see some stimulus-induced increases.

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**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\
**Post date:** [December 22, 2016, 11:07am UTC](https://forum.painresearcher.net/t/time-course-of-light-touch-induced-perk-in-neuropathic-models/139/5 "2016-12-22T11:07:14Z")

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@achamess Here I go with the answers:

It was performed in mice and is not published yet. 7 days after SNI. L3-L5, WB