# TRPV1 Neuromab (N221/17)

**URL:** <https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966>\
**Category:** Uncategorized\
**Created:** [March 14, 2022, 6:53pm UTC](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966 "2022-03-14T18:53:03Z")\
**Posts on this page:** 5\
**Page:** 1

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [March 14, 2022, 6:53pm UTC](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966/1 "2022-03-14T18:53:03Z")

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> **[N221/17](https://dshb.biology.uiowa.edu/N221-17)**
>
> Catalog FieldsAntigen: TrpV1Hybridoma Cells Available: YesAntigen Species: RatDepositor: Trimmer, J.S.Isotype: MIgG1Antigen Sequence: Host Species: mouseDepositors Institution: UC - DavisPositive Tested Species Reactivity: Mouse, RatDepositors Notes:...

@sshiers Do you have thoughts on this TRPV1 antibody? Does it work for mouse and human?

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**Author:** ![sshiers](https://avatars.discourse-cdn.com/v4/letter/s/e9c0ed/32.png) [@sshiers](https://forum.painresearcher.net/u/sshiers)\
**Post date:** [March 14, 2022, 8:49pm UTC](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966/2 "2022-03-14T20:49:08Z")

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I have not tried it - but it does look nice on the datasheet and has KO validation data. They usually do screen for human-reactivity and the data sheet is showing that it is only reactive with mouse and rat so I am guessing it probably wont work on human. Maybe try blasting the immunogen?  
I used this TRPV1 antibody in my JCN paper : [https://www.thermofisher.com/antibody/product/TRPV1-Antibody-Polyclonal/PA1-748](https://www.thermofisher.com/antibody/product/TRPV1-Antibody-Polyclonal/PA1-748)

 ![Figure 10](https://canada1.discourse-cdn.com/flex030/uploads/painresearcher/original/1X/5364b5f7820c0a585d64391d65ad3283c1c6cfc4.jpeg)

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**Author:** ![dmolliver](https://avatars.discourse-cdn.com/v4/letter/d/5e9695/32.png) [@dmolliver](https://forum.painresearcher.net/u/dmolliver)\
**Post date:** [June 22, 2022, 9:01pm UTC](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966/3 "2022-06-22T21:01:08Z")

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Thanks Stephanie, does that antibody work on mouse as well?

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**Author:** ![sshiers](https://avatars.discourse-cdn.com/v4/letter/s/e9c0ed/32.png) [@sshiers](https://forum.painresearcher.net/u/sshiers)\
**Post date:** [June 27, 2022, 3:12pm UTC](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966/4 "2022-06-27T15:12:22Z")

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I havent tried it in mouse

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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [October 29, 2022, 9:48pm UTC](https://forum.painresearcher.net/t/trpv1-neuromab-n221-17/966/5 "2022-10-29T21:48:06Z")

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@sshiers Have you tried the Alomone TRPV1 ab for human tissue?

> **[Anti-TRPV1 (VR1) Antibody](https://www.alomone.com/p/anti-trpv1/ACC-030?attribute_pa_size=50-%25c2%25b5l)**
>
> A highly specific antibody that is designed to recognize TRPV1 from mouse, rat, and human samples. Buy or learn more about our product here.