# Von Frey Apparatus

**URL:** <https://forum.painresearcher.net/t/von-frey-apparatus/1114>\
**Category:** Uncategorized\
**Created:** [July 5, 2023, 7:44pm UTC](https://forum.painresearcher.net/t/von-frey-apparatus/1114 "2023-07-05T19:44:27Z")\
**Posts on this page:** 1\
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**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\
**Post date:** [July 6, 2023, 12:59am UTC](https://forum.painresearcher.net/t/von-frey-apparatus/1114/2 "2023-07-06T00:59:37Z")

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Hi Bishir. Welcome.

Every lab does it a little differently I’ve found, which is one reason for the wide variability seen in the literature.

The most detailed protocol I’ve seen is from Jax, which I think is as good a source as any to imitate since they’re a major mouse breeding and phenotyping site

> **[vonfreytestprocedure.pdf](https://www.jax.org/-/media/jaxweb/files/research-and-faculty/tools-and-resources/peripheral-neuropathy-resource/vonfreytestprocedure.pdf)**
>
> 200.70 KB

You may also consider SUDO from @Boninrp, which is a simpler version of the von Frey assay that has found an audience in recent years. When I use conventional filaments, this is what I do

> **[A simplified up-down method (SUDO) for measuring mechanical nociception in...](https://pubmed.ncbi.nlm.nih.gov/24739328/)**
>
> SUDO thus offers an accurate, fast and user-friendly replacement for the widely used up-down method of Chaplan et al.

We discussed it previously

> [@Simplified Up-Down Method - (SUDO). Anyone use it?](https://forum.painresearcher.net/t/simplified-up-down-method-sudo-anyone-use-it/111):
>
> Has anyone used the Simplified Up-Down method developed by Bonin et al.? It seems to have many benefits, namely speed and simplicity. [image] @fmoehring @ram_kandasamy @LegakisL

Good luck!

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