# Which mouse model most consistently produces pain-like hypersensitivity in your hands? **URL:** \ **Category:** Experimental Techniques\ **Created:** [September 7, 2019, 11:12am UTC](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655 "2019-09-07T11:12:56Z")\ **Posts on this page:** 6\ **Page:** 1
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [September 7, 2019, 11:12am UTC](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655/1 "2019-09-07T11:12:56Z")
In your hands, which mouse model of pain most reliably and strongly produces hypersensitivity in nociceptive assays for you? You can specify which assays in the replies below. Not all models work for all researchers at all places. For me, if I had to pick one of these, I would say SNI produces the most consistent, reproducible and strong hypersensitivity. Most all animals are very sensitive and the surgery is pretty easy to do correctly. And which doesn’t? Which of these models hasn’t produced the expected results? For me, CFA and Chemotherapy have been lackluster. _Poll ([view on site](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655/1))_ @liz @tberta @thicunha @SamineniV @gcorder @ShanTan @esypek @CandlerPaige @jmogil ---
**Author:** ![mamocol](https://avatars.discourse-cdn.com/v4/letter/m/b38774/32.png) [@mamocol](https://forum.painresearcher.net/u/mamocol)\ **Post date:** [September 7, 2019, 2:08pm UTC](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655/2 "2019-09-07T14:08:01Z")
Maybe it’s the chemo dosage? I found high dosage produces very consistent hypersensitivity and lasted for weeks. ---
**Author:** ![thicunha](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/thicunha/32/386_2.png) [@thicunha](https://forum.painresearcher.net/u/thicunha)\ **Post date:** [September 7, 2019, 3:45pm UTC](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655/3 "2019-09-07T15:45:57Z")
for us SNI is also the most reliably model. We also have good results with Paclitaxel using the dose of 8 mg/Kg/each dose four injections each other day. It may last 40 days. We were able to see heat hypoalgesia with this dose. ---
**Author:** ![ShanTan](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/shantan/32/242_2.png) [@ShanTan](https://forum.painresearcher.net/u/ShanTan)\ **Post date:** [September 9, 2019, 3:39pm UTC](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655/4 "2019-09-09T15:39:11Z")
SNI definitely the most reliable. CFA and Formalin are also good, but I’ve had troubles with CFA in producing robust allodynia. ---
**Author:** ![achamess](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/achamess/32/534_2.png) [@achamess](https://forum.painresearcher.net/u/achamess)\ **Post date:** [September 10, 2019, 12:24am UTC](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655/5 "2019-09-10T00:24:42Z")
What kinds of troubles with CFA? Like this? > [@Mechanical threshold testing after CFA in hindpaw -\> Edema in mid hindpaw makes the mice less sensitive?](https://forum.painresearcher.net/t/mechanical-threshold-testing-after-cfa-in-hindpaw-edema-in-mid-hindpaw-makes-the-mice-less-sensitive/122): > > Hi all, So I’m working with the CFA inflammatory model in the hindpaw. I inject 20 ul of CFA subcutaneously. By 24 hours, the hindpaw is very edematous and much larger than the contralateral. I’ve noticed though that when I test von Frey on the mid hindpaw, where it is most swollen, the mice are actually pretty insensitive, which is not what I expect. They seem most sensitive very anteriorly, just behind the toes. Have you noticed this? @ram_kandasamy @LegakisL @tberta @jmogil @fmoehring @thi… ---
**Author:** ![marvizon](https://yyz2.discourse-cdn.com/flex030/user_avatar/forum.painresearcher.net/marvizon/32/211_2.png) [@marvizon](https://forum.painresearcher.net/u/marvizon)\ **Post date:** [September 12, 2019, 6:35pm UTC](https://forum.painresearcher.net/t/which-mouse-model-most-consistently-produces-pain-like-hypersensitivity-in-your-hands/655/6 "2019-09-12T18:35:11Z")
In our hands, CFA produces robust allodynia (von Frey filaments) in both rats and mice. However, I heard that sometimes CFA does not produce a large effect. This may be dependent of the batch of CFA used. The inflammatory component of CFA may vary from batch to batch or may become inactive over time. It may be important to store the CFA in aliquots.