# \#calcium\_imaging
**URL:** https://forum.painresearcher.net/tag/calcium-imaging/1.md
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## [Kappa opioids inhibit spinal output neurons to suppress itch](https://forum.painresearcher.net/t/kappa-opioids-inhibit-spinal-output-neurons-to-suppress-itch/1289)
**Author:** [@PRF-Editorial-Board](https://forum.painresearcher.net/u/PRF-Editorial-Board)\
**Replies:** 0\
**Last updated:** [September 27, 2024, 8:36pm UTC](https://forum.painresearcher.net/t/kappa-opioids-inhibit-spinal-output-neurons-to-suppress-itch/1289 "2024-09-27T20:36:27Z")
Take a look at one of this week’s hand-selected Editor’s Picks and share your thoughts below!
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## [In vitro Model of CFA-induced inflammatory pain-like response](https://forum.painresearcher.net/t/in-vitro-model-of-cfa-induced-inflammatory-pain-like-response/1155)
**Author:** [@vhms0706](https://forum.painresearcher.net/u/vhms0706)\
**Replies:** 3\
**Last updated:** [December 12, 2023, 9:03am UTC](https://forum.painresearcher.net/t/in-vitro-model-of-cfa-induced-inflammatory-pain-like-response/1155 "2023-12-12T09:03:11Z")
Hello! Does anybody know what would be a good in vitro model (in the context of primary DRG culture calcium imaging experiments) of CFA-induced inflammatory pain behavioral model? I have seen some literature doing the …
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## [Population datasets of neural activity in the DRG/dorsal horn?](https://forum.painresearcher.net/t/population-datasets-of-neural-activity-in-the-drg-dorsal-horn/509)
**Author:** [@alex\_naka](https://forum.painresearcher.net/u/alex_naka)\
**Replies:** 2\
**Last updated:** [November 15, 2018, 7:22pm UTC](https://forum.painresearcher.net/t/population-datasets-of-neural-activity-in-the-drg-dorsal-horn/509 "2018-11-15T19:22:30Z")
Hi all, I’m a newcomer to the pain field. Really loving this forum - incredible resource for getting oriented. I’m interested in trying to put together a quantitative model of the circuits/cell-types in the mouse dorsal…
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## [Keeping a spinal cord slice anchored/fixed in a perfusion chamber for calcium imaging](https://forum.painresearcher.net/t/keeping-a-spinal-cord-slice-anchored-fixed-in-a-perfusion-chamber-for-calcium-imaging/116)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\
**Replies:** 5\
**Last updated:** [October 19, 2017, 7:53pm UTC](https://forum.painresearcher.net/t/keeping-a-spinal-cord-slice-anchored-fixed-in-a-perfusion-chamber-for-calcium-imaging/116 "2017-10-19T19:53:30Z")
Does anyone have a good solution for keeping a spinal cord slice (400 um) anchored in place in a perfusion chamber? When I was doing calcium imaging the other day with a slice, throughout the experiment the slice would m…
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## [Quantitative Analysis of Mouse Dural Afferent Neurons Expressing TRPM8, VGLUT3, and NF200](https://forum.painresearcher.net/t/quantitative-analysis-of-mouse-dural-afferent-neurons-expressing-trpm8-vglut3-and-nf200/293)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\
**Replies:** 0\
**Last updated:** [October 7, 2017, 7:34pm UTC](https://forum.painresearcher.net/t/quantitative-analysis-of-mouse-dural-afferent-neurons-expressing-trpm8-vglut3-and-nf200/293 "2017-10-07T19:34:56Z")
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## [Ca++ imaging in DRGs](https://forum.painresearcher.net/t/ca-imaging-in-drgs/220)
**Author:** [@vanja](https://forum.painresearcher.net/u/vanja)\
**Replies:** 3\
**Last updated:** [July 18, 2017, 1:29pm UTC](https://forum.painresearcher.net/t/ca-imaging-in-drgs/220 "2017-07-18T13:29:53Z")
Can anyone suggest a user-friendly method to analyze calcium images in DRG cultures? Perhaps there is already a CellProfiler pipeline people are familiar with? Thanks!!
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## [A threshold problem! Most DRG Sensory Neurons Are Modality-Specific, Not Polymodal](https://forum.painresearcher.net/t/a-threshold-problem-most-drg-sensory-neurons-are-modality-specific-not-polymodal/154)
**Author:** [@lfqueme](https://forum.painresearcher.net/u/lfqueme)\
**Replies:** 0\
**Last updated:** [January 24, 2017, 3:48pm UTC](https://forum.painresearcher.net/t/a-threshold-problem-most-drg-sensory-neurons-are-modality-specific-not-polymodal/154 "2017-01-24T15:48:53Z")
Have you seen the ongoing discussion? As a disclaimer, I consider myself part of the church of the polymodal afferent, thus I found the results difficult to digest. What has come to my attention is that on the …
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## [Recommendations for Calcium Imaging Equipment for DRG cultures and spinal cord slices](https://forum.painresearcher.net/t/recommendations-for-calcium-imaging-equipment-for-drg-cultures-and-spinal-cord-slices/113)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\
**Replies:** 0\
**Last updated:** [October 22, 2016, 1:08am UTC](https://forum.painresearcher.net/t/recommendations-for-calcium-imaging-equipment-for-drg-cultures-and-spinal-cord-slices/113 "2016-10-22T01:08:16Z")
Imagine you're starting a new lab and you need to buy some equipment for calcium imaging in two main applications: DRG cultures and spinal cord slices. What equipment would you get? That is, microscope, camera, perfus…