# \#ihc

**URL:** https://forum.painresearcher.net/tag/ihc/13.md

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## [Sall1 as a microglia marker for IHC or FACS. Anyone tried?](https://forum.painresearcher.net/t/sall1-as-a-microglia-marker-for-ihc-or-facs-anyone-tried/375)

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**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\
**Replies:** 1\
**Last updated:** [January 11, 2018, 2:59pm UTC](https://forum.painresearcher.net/t/sall1-as-a-microglia-marker-for-ihc-or-facs-anyone-tried/375 "2018-01-11T14:59:49Z")

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This paper demonstrates that Sall1, a transcription factor, marks adult microglia specifically in the CNS. They used a transgenic Sall1 reporter mouse to verify. Has anyone done Sall1 IHC with an antibody? @esypek @tbe…

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## [SOX9 as an Astrocyte Marker: Has anyone used in IHC or FACS?](https://forum.painresearcher.net/t/sox9-as-an-astrocyte-marker-has-anyone-used-in-ihc-or-facs/374)

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**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\
**Replies:** 0\
**Last updated:** [January 8, 2018, 10:02pm UTC](https://forum.painresearcher.net/t/sox9-as-an-astrocyte-marker-has-anyone-used-in-ihc-or-facs/374 "2018-01-08T22:02:48Z")

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I saw this thorough paper (Sun et al. 2017) where they identified Sox9 as an astrocyte-specific marker. \[sun2017\_fig2\] Has anyone used this? They use AF3075 from R&D in the paper. Looks pretty good! @tberta