# \#immunohistochemistry **URL:** https://forum.painresearcher.net/tag/immunohistochemistry/4.md [Latest](https://forum.painresearcher.net/latest.md) · [Categories](https://forum.painresearcher.net/categories.md) · [Tags](https://forum.painresearcher.net/tags.md) --- ## [Post-doctoral Research Position in Weir lab- Spinal Cord Group, University of Glasgow](https://forum.painresearcher.net/t/post-doctoral-research-position-in-weir-lab-spinal-cord-group-university-of-glasgow/1469)
**Author:** [@GregW](https://forum.painresearcher.net/u/GregW)\ **Replies:** 0\ **Last updated:** [June 16, 2026, 8:16pm UTC](https://forum.painresearcher.net/t/post-doctoral-research-position-in-weir-lab-spinal-cord-group-university-of-glasgow/1469 "2026-06-16T20:16:50Z")
We have an exciting opportunity for a Research Associate to make a leading contribution to a project entitled “Targeting the primary afferent drivers of neuropathic pain,” working with Dr Greg Weir and his neurobiology o… --- ## [Immunostaining of paraffin-embedded tissues with bone](https://forum.painresearcher.net/t/immunostaining-of-paraffin-embedded-tissues-with-bone/1453)
**Author:** [@wtheodoro](https://forum.painresearcher.net/u/wtheodoro)\ **Replies:** 2\ **Last updated:** [November 4, 2025, 2:05pm UTC](https://forum.painresearcher.net/t/immunostaining-of-paraffin-embedded-tissues-with-bone/1453 "2025-11-04T14:05:39Z")
Hello everyone, I’ve been encountering high background when immunostaining paraffin-embedded, fixed tissues that include both soft and bony components. These specimens are periapical tissues obtained from human teeth d… --- ## [Request to provide a standard method of Mouse DRG culture and Immunocytochemistry Protocol](https://forum.painresearcher.net/t/request-to-provide-a-standard-method-of-mouse-drg-culture-and-immunocytochemistry-protocol/1415)
**Author:** [@Hemanta](https://forum.painresearcher.net/u/Hemanta)\ **Replies:** 0\ **Last updated:** [July 27, 2025, 6:37am UTC](https://forum.painresearcher.net/t/request-to-provide-a-standard-method-of-mouse-drg-culture-and-immunocytochemistry-protocol/1415 "2025-07-27T06:37:28Z")
I am searching for DRG culture and ICC protocol. --- ## [DAB staining Immunohistochemistery](https://forum.painresearcher.net/t/dab-staining-immunohistochemistery/1353)
**Author:** [@HebaReda](https://forum.painresearcher.net/u/HebaReda)\ **Replies:** 2\ **Last updated:** [April 6, 2025, 9:20pm UTC](https://forum.painresearcher.net/t/dab-staining-immunohistochemistery/1353 "2025-04-06T21:20:39Z")
Hi, I am planning to use DAB with nickel in immunohistochemistery so what is the best concentration that should be used please as I am confused with that? I was preparing it freshly by adding 2.7 mg in 10 ml PBS (so a … --- ## [Spinal cord staining with samples isolated from formalin-fixed carcasses](https://forum.painresearcher.net/t/spinal-cord-staining-with-samples-isolated-from-formalin-fixed-carcasses/1108)
**Author:** [@Zoei](https://forum.painresearcher.net/u/Zoei)\ **Replies:** 2\ **Last updated:** [June 30, 2023, 3:48pm UTC](https://forum.painresearcher.net/t/spinal-cord-staining-with-samples-isolated-from-formalin-fixed-carcasses/1108 "2023-06-30T15:48:33Z")
I am working on immunostaining of spinal cord samples that are isolated from carcasses that have been fixed in formalin for a long period of time( about 4-6 month). The staining did not work pretty well( the signal was l… --- ## [Spinal cord staining without fixation](https://forum.painresearcher.net/t/spinal-cord-staining-without-fixation/1101)
**Author:** [@Zoei](https://forum.painresearcher.net/u/Zoei)\ **Replies:** 3\ **Last updated:** [June 30, 2023, 12:25pm UTC](https://forum.painresearcher.net/t/spinal-cord-staining-without-fixation/1101 "2023-06-30T12:25:33Z")
Hello everyone, Has anybody immunostained live spinal cord without fixation? --- ## [HCR RNA-FISH: simple, scalable, superior in situ hybridization. Free HCR RNA-FISH Starter Kits for the Pain Researcher community!](https://forum.painresearcher.net/t/hcr-rna-fish-simple-scalable-superior-in-situ-hybridization-free-hcr-rna-fish-starter-kits-for-the-pain-researcher-community/1000)
**Author:** [@Joyce\_MI](https://forum.painresearcher.net/u/Joyce_MI)\ **Replies:** 2\ **Last updated:** [October 4, 2022, 12:09pm UTC](https://forum.painresearcher.net/t/hcr-rna-fish-simple-scalable-superior-in-situ-hybridization-free-hcr-rna-fish-starter-kits-for-the-pain-researcher-community/1000 "2022-10-04T12:09:18Z")
HCR RNA-FISH: simple, scalable, superior in situ hybridization. Free HCR RNA-FISH Starter Kits for the Pain Researcher community! In situ hybridization is an invaluable tool for neuroscience research, allowing researcher… --- ## [Methodology for counting dorsal horn neurons](https://forum.painresearcher.net/t/methodology-for-counting-dorsal-horn-neurons/804)
**Author:** [@cehanson](https://forum.painresearcher.net/u/cehanson)\ **Replies:** 4\ **Last updated:** [March 4, 2021, 2:02pm UTC](https://forum.painresearcher.net/t/methodology-for-counting-dorsal-horn-neurons/804 "2021-03-04T14:02:47Z")
Hey everyone, I work in a lab that usually studies free nerve endings and DRG, but my project is focused heavily on the dorsal horn. My PI has asked me to: (1) find estimates for the number of neurons in each lamina of … --- ## [Quantitative Analysis of Mouse Dural Afferent Neurons Expressing TRPM8, VGLUT3, and NF200](https://forum.painresearcher.net/t/quantitative-analysis-of-mouse-dural-afferent-neurons-expressing-trpm8-vglut3-and-nf200/293)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [October 7, 2017, 7:34pm UTC](https://forum.painresearcher.net/t/quantitative-analysis-of-mouse-dural-afferent-neurons-expressing-trpm8-vglut3-and-nf200/293 "2017-10-07T19:34:56Z")
--- ## [Parvalbumin antibody - Anyone have suggestions?](https://forum.painresearcher.net/t/parvalbumin-antibody-anyone-have-suggestions/128)
**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\ **Replies:** 0\ **Last updated:** [November 26, 2016, 11:49pm UTC](https://forum.painresearcher.net/t/parvalbumin-antibody-anyone-have-suggestions/128 "2016-11-26T23:49:11Z")
I'm trying to stain for Parvalbumin in DRG neurons. I've been using the Swant Rabbit PV27 antibody, which seems to be the most widely used PV ab for this purpose. I'm using at 1:1000 concentration. Pretty standard IHC co…