# \#retrograde\_labeling

**URL:** https://forum.painresearcher.net/tag/retrograde-labeling/24.md

[Latest](https://forum.painresearcher.net/latest.md) · [Categories](https://forum.painresearcher.net/categories.md) · [Tags](https://forum.painresearcher.net/tags.md)

---

## [DiI labeling and fixation](https://forum.painresearcher.net/t/dii-labeling-and-fixation/982)

<div class="topic-metadata">

**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\
**Replies:** 13\
**Last updated:** [December 22, 2023, 8:26pm UTC](https://forum.painresearcher.net/t/dii-labeling-and-fixation/982 "2023-12-22T20:26:55Z")

</div>

I’ve been trying to retrogradely label DRG neurons from various sites. All the labeling methods have their advantages and drawbacks. DiI is commonly used. I have a 5 mg/ml stock in DMSO. I’ve noticed that it’s kind o…

---

## [Fast Blue Signal Showing up in Multiple Channels](https://forum.painresearcher.net/t/fast-blue-signal-showing-up-in-multiple-channels/1045)

<div class="topic-metadata">

**Author:** [@achamess](https://forum.painresearcher.net/u/achamess)\
**Replies:** 3\
**Last updated:** [March 1, 2023, 9:38am UTC](https://forum.painresearcher.net/t/fast-blue-signal-showing-up-in-multiple-channels/1045 "2023-03-01T09:38:25Z")

</div>

I injected Fast Blue into some mice and recovered the DRG. There is clear FB signal. I am using a Leica epifluorescence microscope with an LED light source and various filters. What I’m noticing is that I am seeing FB …