I want to do some colocalization analysis in the spinal cord. I've done a combo of immunohistochemistry and in situ hybridazation and I have four colors:
DAPI = Blue
White = Gene 1
Red = Gene 2
Green = GFP transgene
I want to quantitate the co-occurrence of these signals, so for example (GFP+/Gene1+/Gene2-) etc in an efficient manner. I've used Photoshop in the past and used the selection too. But I know ImageJ must have a good way to do this. I've shied away from ImageJ in the past because I found it buggy and slow, but I'm willing to use it if it can do what I want. Are there any specific tutorials on how to do? I'm looking around, but the documentation for ImageJ is pretty verbose.
Here's an example of what I'm looking at.
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