New to this site so apologies if this topic has been discussed already
I usually work with rats but have started to incorporate mice into my research recently. Will have to peruse mice soon but have not done it before. I’m well trained in rat perfusion but wanted to ask a few things about mice:
The needle I have to pierce rat heart looks to be too big for mouse. What do people generally use? Is it clamped in place?
Is the needle inserted all the way into the ascending aorta? I do this in rats as it’s quire easy to see, but I imagine it might be more difficult in mice.
What volume of saline and PFA do people use in mice? For rats I generally use 150 ml of each.