RNAscope (In situ hybridization) to study gene expression in pain-related tissues

@esypek Hey, have you used the v2 kit with three colors, including Fluorescein?

Cy3 and C5 look awesome, but Fluorescein is garbage. The issue is definitely the fluor, since we can use the same probe, same tissue, and get wildly different results.

Maybe our fluorescein is bad? Green is never my favorite color for anything.