Does anyone have a good technique for getting RNA from the dorsal horn? I’d like to try to look at dorsal horn gene expression at a couple of spinal levels.
Previously, after removing the DRGs, we tried cutting the spinal cord at the vertebral levels that correlate to specific spinal cord levels, and then slicing that in half coronally with a scalpel to separate the dorsal half from the ventral half. However, since we are doing fresh dissection (not perfused), the cord is very soft by the time we attempt to cut it in half, and it is difficult to reliably divide the spinal cord.
Any advice on how to best dissect this would be extremely helpful!