Experimental Techniques

Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures: Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.) Protocols This category is for the sharing of experimental protocols. Animal Behavior and Models Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research.

Topic	Replies	Views	Activity
About the Experimental Techniques category Experimental Techniques	1	966	December 12, 2017
EF1a promoter to drive expression in DRG in vivo? Reagents and Equipment	14	2416	August 19, 2025
Inquiry on NR2B Primary Antibody for Mouse Fluorescent Immunostaining Experimental Techniques antibody , spinal_cord	1	26	August 14, 2025
Transgenic Cre mouse for labeling periphery nerves Experimental Techniques	2	83	August 11, 2025
Request to provide a standard method of Mouse DRG culture and Immunocytochemistry Protocol Microscopy (IHC/ISH/ICC/In Vivo) drg , immunohistochemistry , spinal_cord	0	48	July 27, 2025
Housekeeping proteins for western blot that do not change following nerve injury Experimental Techniques	3	67	July 9, 2025
Ideal age to record PSCs from mice spinal cord without stimulation electrode Experimental Techniques	2	60	April 16, 2025
DAB staining Immunohistochemistery Microscopy (IHC/ISH/ICC/In Vivo) immunohistochemistry	2	46	April 6, 2025
Frozen vs. Non-frozen IHC (Fixed) Spinal Cords. Thoughts? Experimental Techniques	13	3338	March 17, 2025
Establishment of a Magnetically Controlled Scalable Nerve Injury Model Animal Behavior and Models	0	24	September 20, 2024
Protein Isolation for Western Blots (protocols)? Experimental Techniques	3	507	July 30, 2024
Pgp9.5 glabrous skin staining Protocols	20	5923	July 10, 2024
P2X3 Antibodies Reagents and Equipment	7	211	June 19, 2024
Are there dyes to label neurons, similar to how SR101 labels astrocytes in 20 minutes under 2P? Microscopy (IHC/ISH/ICC/In Vivo) spinal_cord	0	140	May 30, 2024
Intraganglionic injections into mouse DRG? Protocols	20	6938	May 8, 2024
Optimizing Electronic Von Frey for Mouse Experimental Techniques	1	213	April 11, 2024
Experience with electronic von Frey Animal Behavior and Models	1	236	March 29, 2024
Experience with IB4-Saporin? Experimental Techniques	5	267	February 1, 2024
DRG neuron adherence issue in culture Protocols	2	435	January 27, 2024
DiI labeling and fixation Experimental Techniques drg , retrograde_labeling	13	1861	December 22, 2023
Precellys 24 for homogenization of mouse dorsal root ganglia Experimental Techniques	7	657	December 20, 2023
Whole mount DRG staining Experimental Techniques	10	949	December 13, 2023
In vitro Model of CFA-induced inflammatory pain-like response Experimental Techniques calcium_imaging	3	427	December 12, 2023
Death after intrathecal capsaicin? Experimental Techniques	6	369	December 1, 2023
Topical application of capsaicin to mouse skin Animal Behavior and Models	2	331	December 1, 2023
Con/Fon labeling in DRG Reagents and Equipment viral_vectors	3	299	October 8, 2023
Protocol for dissection and culture of murine dorsal root ganglia neurons Protocols	0	374	August 6, 2023
How to prepare 3% carrageenan? Reagents and Equipment	4	378	July 23, 2023
Spinal cord staining with samples isolated from formalin-fixed carcasses Experimental Techniques spinal_cord , immunohistochemistry	2	293	June 30, 2023
Spinal cord staining without fixation Protocols spinal_cord , immunohistochemistry	3	331	June 30, 2023