Experimental Techniques

Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research. Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.) Protocols This category is for the sharing of experimental protocols. Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures: Animal Behavior and Models

Topic	Replies	Views	Activity
About the Experimental Techniques category Experimental Techniques	1	1008	December 12, 2017
Alternative to TUBB3 (TUJ1) for axon staining, non-neuronal staining Reagents and Equipment	2	1388	April 13, 2026
Immunostaining of paraffin-embedded tissues with bone Protocols antibody , immunohistochemistry	2	120	November 4, 2025
RNAscope (In situ hybridization) to study gene expression in pain-related tissues Experimental Techniques	88	21910	October 23, 2025
Pain behaviors in face Experimental Techniques	0	145	October 15, 2025
Tone-pain conditioning Protocols	2	150	September 25, 2025
Human on Human high IgG background on human DRG/nerve Experimental Techniques	4	617	September 5, 2025
EF1a promoter to drive expression in DRG in vivo? Reagents and Equipment	14	2611	August 19, 2025
Inquiry on NR2B Primary Antibody for Mouse Fluorescent Immunostaining Experimental Techniques antibody , spinal_cord	1	94	August 14, 2025
Transgenic Cre mouse for labeling periphery nerves Experimental Techniques	2	224	August 11, 2025
Request to provide a standard method of Mouse DRG culture and Immunocytochemistry Protocol Microscopy (IHC/ISH/ICC/In Vivo) drg , immunohistochemistry , spinal_cord	0	117	July 27, 2025
Housekeeping proteins for western blot that do not change following nerve injury Experimental Techniques	3	181	July 9, 2025
Ideal age to record PSCs from mice spinal cord without stimulation electrode Experimental Techniques	2	142	April 16, 2025
DAB staining Immunohistochemistery Microscopy (IHC/ISH/ICC/In Vivo) immunohistochemistry	2	137	April 6, 2025
Frozen vs. Non-frozen IHC (Fixed) Spinal Cords. Thoughts? Experimental Techniques	13	3634	March 17, 2025
Establishment of a Magnetically Controlled Scalable Nerve Injury Model Animal Behavior and Models	0	49	September 20, 2024
Protein Isolation for Western Blots (protocols)? Experimental Techniques	3	611	July 30, 2024
Pgp9.5 glabrous skin staining Protocols	20	6331	July 10, 2024
P2X3 Antibodies Reagents and Equipment	7	306	June 19, 2024
Are there dyes to label neurons, similar to how SR101 labels astrocytes in 20 minutes under 2P? Microscopy (IHC/ISH/ICC/In Vivo) spinal_cord	0	173	May 30, 2024
Intraganglionic injections into mouse DRG? Protocols	20	7237	May 8, 2024
Optimizing Electronic Von Frey for Mouse Experimental Techniques	1	299	April 11, 2024
Experience with electronic von Frey Animal Behavior and Models	1	320	March 29, 2024
Experience with IB4-Saporin? Experimental Techniques	5	331	February 1, 2024
DRG neuron adherence issue in culture Protocols	2	572	January 27, 2024
DiI labeling and fixation Experimental Techniques drg , retrograde_labeling	13	2121	December 22, 2023
Precellys 24 for homogenization of mouse dorsal root ganglia Experimental Techniques	7	756	December 20, 2023
Whole mount DRG staining Experimental Techniques	10	1276	December 13, 2023
In vitro Model of CFA-induced inflammatory pain-like response Experimental Techniques calcium_imaging	3	516	December 12, 2023
Death after intrathecal capsaicin? Experimental Techniques	6	491	December 1, 2023