Experimental Techniques

Protocols This category is for the sharing of experimental protocols. Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research. Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.) Animal Behavior and Models Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures:

Topic	Replies	Views	Activity
About the Experimental Techniques category Experimental Techniques	1	963	December 12, 2017
Protein Isolation for Western Blots (protocols)? Experimental Techniques	2	443	July 19, 2024
Pgp9.5 glabrous skin staining Protocols	20	5532	July 10, 2024
P2X3 Antibodies Reagents and Equipment	7	182	June 19, 2024
Are there dyes to label neurons, similar to how SR101 labels astrocytes in 20 minutes under 2P? Microscopy (IHC/ISH/ICC/In Vivo) spinal_cord	0	120	May 30, 2024
Intraganglionic injections into mouse DRG? Protocols	20	6726	May 8, 2024
Optimizing Electronic Von Frey for Mouse Experimental Techniques	1	154	April 11, 2024
Experience with electronic von Frey Animal Behavior and Models	1	166	March 29, 2024
Experience with IB4-Saporin? Experimental Techniques	5	237	February 1, 2024
DRG neuron adherence issue in culture Protocols	2	313	January 27, 2024
DiI labeling and fixation Experimental Techniques drg , retrograde_labeling	13	1595	December 22, 2023
Precellys 24 for homogenization of mouse dorsal root ganglia Experimental Techniques	7	603	December 20, 2023
Whole mount DRG staining Experimental Techniques	10	696	December 13, 2023
In vitro Model of CFA-induced inflammatory pain-like response Experimental Techniques calcium_imaging	3	364	December 12, 2023
Death after intrathecal capsaicin? Experimental Techniques	6	285	December 1, 2023
Topical application of capsaicin to mouse skin Animal Behavior and Models	2	272	December 1, 2023
EF1a promoter to drive expression in DRG in vivo? Reagents and Equipment	6	2208	November 19, 2023
Con/Fon labeling in DRG Reagents and Equipment viral_vectors	3	281	October 8, 2023
Protocol for dissection and culture of murine dorsal root ganglia neurons Protocols	0	361	August 6, 2023
How to prepare 3% carrageenan? Reagents and Equipment	4	321	July 23, 2023
Spinal cord staining with samples isolated from formalin-fixed carcasses Experimental Techniques spinal_cord , immunohistochemistry	2	279	June 30, 2023
Spinal cord staining without fixation Protocols spinal_cord , immunohistochemistry	3	297	June 30, 2023
Best AAV serotype for transduction of spinal neurons in the dorsal horn? Reagents and Equipment	5	1622	May 24, 2023
Analyzing brain slice images produced by slide scanner Experimental Techniques	1	231	May 15, 2023
Best storage method for peripheral nerves? Protocols	2	293	May 15, 2023
Cre-inducible mice - reporter to tag the nucleus? Reagents and Equipment	10	429	April 26, 2023
Calcium imaging in spinal cord lumbar region using patch code Experimental Techniques	0	251	April 10, 2023
Digital vs real-time for qPCR Reagents and Equipment	3	420	March 27, 2023
RNAscope (In situ hybridization) to study gene expression in pain-related tissues Experimental Techniques	87	20189	March 20, 2023
Fast Blue Signal Showing up in Multiple Channels Experimental Techniques retrograde_labeling	3	399	March 1, 2023