Experimental Techniques

Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures: Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.) Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research. Protocols This category is for the sharing of experimental protocols. Animal Behavior and Models

Topic	Replies	Views	Activity
About the Experimental Techniques category Experimental Techniques	1	978	December 12, 2017
Immunostaining of paraffin-embedded tissues with bone Protocols antibody , immunohistochemistry	2	54	November 4, 2025
RNAscope (In situ hybridization) to study gene expression in pain-related tissues Experimental Techniques	88	21229	October 23, 2025
Pain behaviors in face Experimental Techniques	0	75	October 15, 2025
Tone-pain conditioning Protocols	2	94	September 25, 2025
Human on Human high IgG background on human DRG/nerve Experimental Techniques	4	530	September 5, 2025
EF1a promoter to drive expression in DRG in vivo? Reagents and Equipment	14	2487	August 19, 2025
Inquiry on NR2B Primary Antibody for Mouse Fluorescent Immunostaining Experimental Techniques antibody , spinal_cord	1	52	August 14, 2025
Transgenic Cre mouse for labeling periphery nerves Experimental Techniques	2	134	August 11, 2025
Request to provide a standard method of Mouse DRG culture and Immunocytochemistry Protocol Microscopy (IHC/ISH/ICC/In Vivo) drg , immunohistochemistry , spinal_cord	0	77	July 27, 2025
Housekeeping proteins for western blot that do not change following nerve injury Experimental Techniques	3	103	July 9, 2025
Ideal age to record PSCs from mice spinal cord without stimulation electrode Experimental Techniques	2	90	April 16, 2025
DAB staining Immunohistochemistery Microscopy (IHC/ISH/ICC/In Vivo) immunohistochemistry	2	65	April 6, 2025
Frozen vs. Non-frozen IHC (Fixed) Spinal Cords. Thoughts? Experimental Techniques	13	3468	March 17, 2025
Establishment of a Magnetically Controlled Scalable Nerve Injury Model Animal Behavior and Models	0	30	September 20, 2024
Protein Isolation for Western Blots (protocols)? Experimental Techniques	3	537	July 30, 2024
Pgp9.5 glabrous skin staining Protocols	20	6081	July 10, 2024
P2X3 Antibodies Reagents and Equipment	7	234	June 19, 2024
Are there dyes to label neurons, similar to how SR101 labels astrocytes in 20 minutes under 2P? Microscopy (IHC/ISH/ICC/In Vivo) spinal_cord	0	151	May 30, 2024
Intraganglionic injections into mouse DRG? Protocols	20	7017	May 8, 2024
Optimizing Electronic Von Frey for Mouse Experimental Techniques	1	241	April 11, 2024
Experience with electronic von Frey Animal Behavior and Models	1	269	March 29, 2024
Experience with IB4-Saporin? Experimental Techniques	5	288	February 1, 2024
DRG neuron adherence issue in culture Protocols	2	477	January 27, 2024
DiI labeling and fixation Experimental Techniques drg , retrograde_labeling	13	1981	December 22, 2023
Precellys 24 for homogenization of mouse dorsal root ganglia Experimental Techniques	7	687	December 20, 2023
Whole mount DRG staining Experimental Techniques	10	1076	December 13, 2023
In vitro Model of CFA-induced inflammatory pain-like response Experimental Techniques calcium_imaging	3	453	December 12, 2023
Death after intrathecal capsaicin? Experimental Techniques	6	421	December 1, 2023
Topical application of capsaicin to mouse skin Animal Behavior and Models	2	365	December 1, 2023