Experimental Techniques

Protocols This category is for the sharing of experimental protocols. Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures: Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research. Animal Behavior and Models Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.)

Topic	Replies	Views	Activity
About the Experimental Techniques category Experimental Techniques	1	635	December 12, 2017
Derivation of dorsal spinal sensory interneurons from human pluripotent stem cells Experimental Techniques	0	14	February 27, 2021
Methodology for counting dorsal horn neurons Experimental Techniques immunohistochemistry , spinal_cord	1	27	February 26, 2021
RNAscope (In situ hybridization) to study gene expression in pain-related tissues Experimental Techniques	71	12112	February 18, 2021
Tamoxifen - injection/ recombination timeframe Reagents and Equipment	5	62	February 15, 2021
DRGs sectioning Reagents and Equipment	3	87	February 11, 2021
Preservation of postnatal mouse spinal cord sections for RNAScope Microscopy (IHC/ISH/ICC/In Vivo) spinal_cord	7	118	February 3, 2021
Mounting many DRG for cryosectioning - share your tips Experimental Techniques	12	1604	January 30, 2021
Protein Isolation for Western Blots (protocols)? Experimental Techniques	1	72	January 18, 2021
Human on Human high IgG background on human DRG/nerve Experimental Techniques	2	44	January 10, 2021
Looking for a good CGRP antibody (specific staining, low background) Reagents and Equipment	38	3095	January 10, 2021
Transgenic Cre or other reporter lines for specific labeling of sensory, motor and autonomic neurons in the periphery Experimental Techniques	7	785	January 8, 2021
Cre recombinase antibody Reagents and Equipment antibody	1	157	December 18, 2020
FACS spinal cord microglia Experimental Techniques	8	856	December 4, 2020
Raster plots for thermal nociception tests Animal Behavior and Models	1	95	November 14, 2020
Viral delivery targeting microglia Experimental Techniques	4	216	September 14, 2020
Mounting multiple mouse spinal cords for cryosectioning - How do you do it? Experimental Techniques	1	227	September 13, 2020
Protocol for mouse DRG protein lysate preparation Experimental Techniques	7	158	September 12, 2020
Rapid Isolation of Dorsal Root Ganglion Macrophages \| Protocol Experimental Techniques	0	117	September 9, 2020
Tips for spinal cord slices with dorsal roots attached? Electrophysiology	11	415	September 8, 2020
A video protocol for rapid dissection of mouse dorsal root ganglia from Protocols	0	127	September 8, 2020
Compartmentalized DRG cultures in Xona Microfluid Chambers Experimental Techniques	0	112	September 4, 2020
Immunohistochemistry/Immunofluorescence Protocols for PNS tissues - share yours Experimental Techniques	0	126	September 3, 2020
Recommendation for ATF3 antibody (not Santa Cruz) Reagents and Equipment	10	520	August 28, 2020
Acclimation Protocols for Nociceptive Assays - How long and how often? Animal Behavior and Models	2	272	July 17, 2020
Best "supraspinal" nociceptive assays? Animal Behavior and Models	4	738	July 17, 2020
Quantifying DRG neuronal death after axotomy Experimental Techniques	5	218	June 24, 2020
Wireless EMG in rodents? Experimental Techniques	0	184	June 24, 2020
Pain semantics study. HELP Experimental Techniques	0	193	May 4, 2020
Performing batteries of nociceptive assays same day Animal Behavior and Models	8	441	March 22, 2020