Experimental Techniques

Reagents and Equipment Use this category to discuss reagents and equipment that are used in your pain research. Microscopy (IHC/ISH/ICC/In Vivo) This category is for microscopy and related procedures: Electrophysiology This category is for all types of electrophysiology (patch-clamp, in vivo, field potentials, etc.) Animal Behavior and Models Protocols This category is for the sharing of experimental protocols.

Topic	Replies	Views	Activity
About the Experimental Techniques category Experimental Techniques	1	1009	December 12, 2017
Probelm with Patch clamp of Dissociated DRG neurons Electrophysiology	7	1166	September 17, 2026
Alternative to TUBB3 (TUJ1) for axon staining, non-neuronal staining Reagents and Equipment	2	1418	April 13, 2026
Immunostaining of paraffin-embedded tissues with bone Protocols antibody , immunohistochemistry	2	140	November 4, 2025
RNAscope (In situ hybridization) to study gene expression in pain-related tissues Experimental Techniques	88	21962	October 23, 2025
Pain behaviors in face Experimental Techniques	0	161	October 15, 2025
Tone-pain conditioning Protocols	2	169	September 25, 2025
Human on Human high IgG background on human DRG/nerve Experimental Techniques	4	638	September 5, 2025
EF1a promoter to drive expression in DRG in vivo? Reagents and Equipment	14	2638	August 19, 2025
Inquiry on NR2B Primary Antibody for Mouse Fluorescent Immunostaining Experimental Techniques antibody , spinal_cord	1	111	August 14, 2025
Transgenic Cre mouse for labeling periphery nerves Experimental Techniques	2	243	August 11, 2025
Request to provide a standard method of Mouse DRG culture and Immunocytochemistry Protocol Microscopy (IHC/ISH/ICC/In Vivo) drg , immunohistochemistry , spinal_cord	0	133	July 27, 2025
Housekeeping proteins for western blot that do not change following nerve injury Experimental Techniques	3	204	July 9, 2025
Ideal age to record PSCs from mice spinal cord without stimulation electrode Experimental Techniques	2	160	April 16, 2025
DAB staining Immunohistochemistery Microscopy (IHC/ISH/ICC/In Vivo) immunohistochemistry	2	157	April 6, 2025
Frozen vs. Non-frozen IHC (Fixed) Spinal Cords. Thoughts? Experimental Techniques	13	3663	March 17, 2025
Establishment of a Magnetically Controlled Scalable Nerve Injury Model Animal Behavior and Models	0	50	September 20, 2024
Protein Isolation for Western Blots (protocols)? Experimental Techniques	3	616	July 30, 2024
Pgp9.5 glabrous skin staining Protocols	20	6362	July 10, 2024
P2X3 Antibodies Reagents and Equipment	7	313	June 19, 2024
Are there dyes to label neurons, similar to how SR101 labels astrocytes in 20 minutes under 2P? Microscopy (IHC/ISH/ICC/In Vivo) spinal_cord	0	173	May 30, 2024
Intraganglionic injections into mouse DRG? Protocols	20	7254	May 8, 2024
Optimizing Electronic Von Frey for Mouse Experimental Techniques	1	304	April 11, 2024
Experience with electronic von Frey Animal Behavior and Models	1	320	March 29, 2024
Experience with IB4-Saporin? Experimental Techniques	5	335	February 1, 2024
DRG neuron adherence issue in culture Protocols	2	588	January 27, 2024
DiI labeling and fixation Experimental Techniques drg , retrograde_labeling	13	2140	December 22, 2023
Precellys 24 for homogenization of mouse dorsal root ganglia Experimental Techniques	7	780	December 20, 2023
Whole mount DRG staining Experimental Techniques	10	1310	December 13, 2023
In vitro Model of CFA-induced inflammatory pain-like response Experimental Techniques calcium_imaging	3	527	December 12, 2023