Ideal age to record PSCs from mice spinal cord without stimulation electrode

Experimental Techniques
1

Hello!

I have two queries regarding ephys from spinal cord slice,

  1. For recording of Post synaptic currents, is there any method other than stimulation electrode to record the PSCs in mice spinal cord in P0-P12 mice spinal cord ?

Also, what is the perfect age of mice to record PSCs from spinal cord slices?

** I have recorded for 1h 10 min from P4 mice spinal cord, with only 52 events without stimulation electrode.

  1. with Leica vibratome VT1200S, is there any better parameter to optimize the protocol to get more synaptic events?

** I use 0.50 mm/s, 1.00 mm ampl and 400um slice thickness.

Best regards,
Anutosh