Protein Isolation for Western Blots (protocols)?

Hey all,
Looking to do some protein work soon. I have some personal protocols from grad school but it’d be nice to see what people are doing.

If you’re willing, share your protocol for:

Mouse

  • Spinal cord
  • DRG
  • Nerve

Human

  • DRG
  • Nerve

I made video a few years back on Mouse DRG lysate prep based on the protocol @tberta taught me

I used to use a BeadBeater as well for many things. I like to avoid the sonicator whenever possible, but I don’t have the bead beater anymore.

@tberta @sshiers @CandlerPaige @thicunha @ShanTan