What’s the fastest anyone can dissociate DRGs enzymatically? What enzyme mix and conditions? Any literature refs? The goal is to get from whole ganglia to dissociated, healthy DRGs for FACS in as short a time as possible.
Not sure what the protocol is on bumping discussions but I’d be very interested in more information on this subject? Or alternatively a protocol specifically for nuclear preps from DRG?
Hi. welcome to the forum. Bumping is totally fine. Unfortunately, I don’t have a great answer for you since nuclei isolation from DRG has not been done a whole lot in the literature yet.
A few references:
Good luck! Keep us posted.