I’m needing to inject viral vectors directly into mouse DRG so that I can localize the expression just to the DRG. I can get very good expression when I inject intrathecally, but that enables the spread of the virus to parts of the CNS where I don’t want it to go.
So I need to do an intra-DRG injection. I know it can be done. A recent paper by Vicuna et al. in Nat. Med used intraganglionic injection to control transgene expression. And I know it can be done in the rat, but for mouse, I don’t have a detailed protocol.
I know that I will need to expose a DRG (lumbar, L4 probably), which will require some kind of laminectomy or small bone removal. It’d be great if anyone has some in-house protocols with images/video to help me perform this procedure. Also, if you’ve injected virus, some info on the volume and titer would be great.
@tberta do you have any thoughts on this?