In vivo dynamics of AAV-mediated gene delivery to sensory neurons of the

[achamess]

https://www.nature.com/articles/s41598-017-01004-y

[achamess]

This is useful and pretty rigorous. Looks like AAV1 is best from the TG. That jives well with what I found recently from the paw, although I still need to quantify.

Stuff I tried during grad school/post-doc that won't get published but could be useful

AAV1-hSyn-Cre retrogradely infects DRG neurons from the paw. Mouse: Ai32 (R26-CAG-LSL-ChR2) Titer: ~1E13 -> 10 ul injected To be determined: Percentage of neurons infected Does it jump to spinal neurons anterogradely This behavior is 3 weeks post infection. At 1 week, there was no behavior. Also, at same time period, retro-AAV-Cre injected into the same mouse line did not produce any behavior. So, I’m not optimistic about retro-AAV for mouse paw.

@tberta @YongHo