Stuff I tried during grad school/post-doc that won't get published but could be useful

Resources
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AAV1-hSyn-Cre retrogradely infects DRG neurons from the paw.
Mouse: Ai32 (R26-CAG-LSL-ChR2)
Titer: ~1E13 -> 10 ul injected

To be determined:

  • Percentage of neurons infected
  • Does it jump to spinal neurons anterogradely

This behavior is 3 weeks post infection. At 1 week, there was no behavior.

Also, at same time period, retro-AAV-Cre injected into the same mouse line did not produce any behavior. So, I’m not optimistic about retro-AAV for mouse paw.